Day 1 :
- Track 1: Novel Approaches to Analytical and Bioanalytical Methods
Track:2 Analytical Methodology
Session Introduction
Seda Nur Topkaya
Ege University School of Pharmacy, Turkey
Title: Electrochemical DNA biosensors and their potential applications to nanobiotechnology
Biography:
Seda Nur Topkaya has completed his PhD in 2013 at Analytical Chemistry from Faculty of Pharmacy, Ege University, Turkey. She also conducted her PhD researches at Harvard and MIT for 1 year about tissue engineering. Her main research interests are electrochemistry, electrochemical based DNA biosensors, detection of drug-DNA applications and also 2D-3D tissue engineering. She has published more than 10 papers in reputed journals and serving as a reviewer of many international journals.
Abstract:
Electrochemical based biosensors have been recently recognized as potential candidates for the requirements of nanotechnology applications. Sensitive and rapid detection of very few amounts of target nucleic acids (DNA or RNA) in biological matrices has attracted considerable attention from many fields, such as clinical diagnosis, drug researches and environmental analysis. Electrochemical DNA biosensors offer a highly sensitive and promising method for the detection of hybridization, genetic polymorphisms and mutations, alterations of genes and potential drug-DNA interactions because of their short assay time, miniaturization, portability, and low-cost. The main principle of electrochemical DNA biosensors is based on the conversion of hybridization events into the analytical signals via a transducer. The most common way is the direct detection of DNA oxidation signals of guanine bases through voltammetric techniques by evaluating the intrinsic signal changes of bases. Specific DNA/RNA hybridization can also be monitored using selective redox indicators, amperometric techniques, nanomaterials or electrochemical impedance measurements indirectly. Electrochemical based detection methods meet the sensitivity requirements with its picomolar detection limit in real samples and selective for the target DNA/RNA.
Istvan Halasz
PQ Corporation, USA
Title: Precise analysis of elements in silica powders by LA-ICP-MS
Biography:
Istvan Halasz obtained his PhD in Hungary from the Hungarian Hydrocarbon Institute, where he developed and scaled-up efficient processes for pharmaceutical, fine chemical and petrochemical industries. Later, at the Hungarian Academy of Sciences and at USA Universities, he studied zeolite catalysis, oxide superconductor synthesis, and catalytic fume abatement for automobile exhausts. In the past 16 years, he has investigated the properties of silicates at PQ R&D. He chaired the Philadelphia Catalysis Club; is current President of North-East Corridor Zeolite Association (NECZA); edited a book on catalysis, and authored 110+ book chapters and papers, 7 patents, and 80+ conference presentations.
Abstract:
Silica is important component of many heterogeneous catalysts. Catalysis affects ~90% of all chemical products. For elemental analysis by AA or ICP these silica-based catalysts are dissolved by HF and other acids. HF is exceptionally dangerous because of its ability to diffuse through the skin where the fluoride ion binds calcium with subsequent disruption of electrical activity. Therefore, it is desirable to minimize its use to reduce risk and eliminate a lengthy and tedious dissolution process. We speculated that coupling laser ablation (LA) to an ICP-MS could fulfill this desire. However, LA cannot be used for powder samples and LA-ICP-MS is known to be much less accurate than liquid phase measurements, owing to inhomogeneous ablated particle size and inadequate analysis parameters. Here, we report the development of a LA-ICP-MS method for accurately analyzing powdered silica supported catalysts. We melt the powders with a mixture of Li2B4O7 - LiBO2 into a homogeneous solid bead, vaporize the surface with a laser, and then apply a small cyclone before the ICP. Moreover, we optimized the analysis conditions by using statistical experimental design of 11 parameters. Using three commercial zeolite catalysts having Si/Al ratios 2.6, 40, and 140, we show that different parameters significantly affect the accuracy of measuring their Al contents. The relative standard deviation, RSD, remains <5% over the entire concentration range tested, sometimes even <0.5%, which is better than that obtained by the HF dissolution technique.
Radim Vespalec
Academy of Sciences of the Czech Republic, Czech Republic
Title: Analytical tasks stemming from therapeutical prospects of electron deficient boron cluster compounds
Biography:
Radim Vespalec has received equivalent to PhD from the Institute of Physical Chemistry, Academy of Sciences of the Czechoslovak Republic, Praha, in 27. He received the scientific degree from Technical University of Pardubice, and the pedagogical Assoc. Prof. degree from Masaryk University Brno. He is Senior Scientist in the Institute of Biophysics. Web of Science reports his 80 scientific articles and also he has contributed to 3 monographs
Abstract:
The existence, reactions, structures and properties of compounds occurring in nature, and their synthetic analogues are explainable by the idea of two-center two-electron bond. These families contain either electron exact or electron rich building blocks from the viewpoint of electron structure. Electron deficient building blocks have never been found in nature, and exist only in synthetic species. Boron cluster compounds (BCCs) create the most intensely investigated family of species with electron deficient cluster. Their existence has been explained by the accumulation of unique electron deficient bonds, which bind together three boron atoms or, sometimes, their substitutes, in clusters. Pronouncedly electron deficient clusters either determine or substantially affect properties of BCCs, and their prospects. Therapeutical prospects attract the highest attention now, and many compounds with boron clusters are synthesized as candidates for therapeutical uses. These compounds must pass through mandatory studies and checks, which require variety of chemical analyses, identically with other compounds. However, analytical methods do not exist for analyses of compounds with boron clusters. The pieces of knowledge from chiral separation of BCCs prove the dissimilarity of some analytical properties of species with and without boron clusters, and indicate the absence of criteria for the a priori estimation of different analytical properties for compounds with and without clusters. Thus, missing analytical methods cannot be derived from existing knowledge. Analytical research of BCCs motivated by their medical prospects is the best way to preventive elimination some obstacles, which may hamper medical uses of compounds with boron clusters.
Tudor Arvinte
University of Geneva, Switzerland
Title: New analytical methods to measure protein aggregates in biopharmaceuticals
Biography:
Tudor Arvinte, PhD received his academic training in physics at the University of Jassy, Romania, and his PhD in biophysics from the University of Düsseldorf, Germany. He performed his Doctoral work and Postdoctoral stage at the Max-Planck-Institute West Germany and held numerous research positions in Europe and the USA: at C.N.R.S., Orléans, France, at Cornell University, New York, at Texas A&M University, and at the Biophor Corporation, College Station, Texas, USA. In 1989 he joined Ciba-Geigy Pharmaceuticals in Horsham, England, and in 1994 he moved to Ciba- Geigy in Basel, Switzerland. Until 2002 he worked as Head of Exploratory Formulation, Novartis Biotechnology Development & Production, Basel. He worked on the characterization and formulation of more than 130 protein and peptide drugs. He has over 80 publications and holds 13 patents on formulations of proteins: one patented formulation for hirudin is used in the marketed product. Since 2001 he is invited Professor at the School of Pharmacy, University of Geneva, Switzerland where he is teaching a post-graduate course on “Formulation and delivery of protein biopharmaceuticals”. He is also Visiting Professor at the Department of Pharmacy, School of Health and Life Sciences King’s College London, UK. In 2003 he co-founded Therapeomic, Inc., a biotech company focused on developing formulations for biopharmaceuticals in collaborations with pharmaceutical companies.
Abstract:
A good characterisation of the protein aggregation states and of the presence of particulate matter in biopharmaceuticals can only be achieved through a convergence of evidence from numerous classical and orthogonal analytical methods. Based on case studies, different new orthogonal methods will be presented. No single method can be used as an absolute characterisation of the aggregation states. Only by using together many different, complementary methods we can obtain reliable conclusions and reveal an abroad picture on the protein aggregation states and the presence of particulate matter in biopharmaceuticals.
Syed N Alvi
King Faisal Specialist Hospital & Research Center, Saudi Arabia
Title: Determination of amlodipine in human plasma by liquid chromatography–tandem mass spectrometry and its application to pharmacokinetic & bioequivalence studies
Biography:
Syed N Alvi obtained his PhD in Chemistry from Osmania University, Hyderabad, India in 2001. He is currently Scientist at King Faisal Specialist Hospital & Research Centre, Riyadh, Saudi Arabia. He his research interest includes method development and validation and application for pharmacokinetic and bioequivalence studies.
Abstract:
A rapid Liquid Chromatographic-Tandem Mass Spectrometric (LC-MS/MS) assay for the measurement of amlodipine level in human plasma was developed and validated. Amlodipine and tizanidine (IS) were extracted from plasma using mixture of dichloromethane and tertiary butyl methyl ether (1:3, v:v) and reconstituted with 100 µl mixture of methanol and water (1:1, v:v). The mobile phase consisted of acetonitrile and 0.1% formic acid (80:20, v:v). Analysis was performed at room temperature using a reversed phase Atlantis dC18 (2.1x100 mm, 3 µm) column. The components of interest were detected in the positive ion mode of electrospray ionization using transition 409.8 → 238.4 and 254.3 → 43.9 for amlodipine and the IS, respectively. The relationship between amlodipine concentration in plasma and peak height ratio of amlodipine to IS was linear 0.9868 in the range of 0.2–20 ng/ml; intra and inter-day accuracy between 101-114%, and coefficient of variations were ≤14.4%. The quantification limit of amlodipine in 0.5 ml plasma was 0.2 ng/ml and the detection limit was 0.1 ng/ml. The method was successfully validated and applied in a bioequivalence study of four tablet formulations of amlodipine.
Florence Geneste
University of Rennes ,France
Title: Flow electrochemical sensor for trace analysis of heavy metals
Biography:
Florence Geneste was graduated from the Ecole Supérieure de Chimie Physique Electronique de Lyon. She completed her PhD in Chemistry in 1996 at the University of Paris-Sud. She worked as a Postdoctoral researcher in Thomson-CSF (Orsay) and then at the University of Cambridge, with Prof. A.B. Holmes from 1997 to 1999. She joined the University of Rennes as Associate Director of Research CNRS in 1999. Her research interest is focused on the modification of porous electrodes for application in flow electrochemistry. She is involved in the development of novel analytical methods for sensors and biosensors applications and in supported catalysis.
Abstract:
Flow electrochemical sensors are highly effective to enhance mass transport when a pre-concentration step of the analyte is required to achieve trace analysis. They are advantageous because they are easily automatable and allow approaching the ideal real-time analysis. Porous electrodes are relevant to flow electrochemical systems due to their good hydrodynamic properties and their high specific surface area. In this work, we will present the use of graphite felt as electrode material for trace analysis of heavy metals in flow systems. Its properties combined to a well-suited flow electrochemical cell give rise to efficient accumulation step in anodic stripping voltammetry. Since this material is easy to modify by electro grafting methods, selective analyses can be performed thanks to convenient receptors covalently attached on the surface of the fibers. Electro grafting methods involving reduction of diazonium salts and oxidation of amines in organic and aqueous media were used to prepare methoxy, carboxylate and cyclam-modified electrodes. The efficiency of the immobilization methods was checked by cyclic voltammetry using redox probes and by XPS analyses. The performances of the modified electrodes for trace analysis of copper were then evaluated in terms of sensitivity and selectivity. The influence of the electro grafting method and the nature of the linker on the electrochemical signal obtained by linear sweep stripping voltammetry analysis after a preconcentration step performed at open circuit was highlighted.
Li Niu
Chinese Academy of Sciences, China
Title: R&D of electrochemical sensors & instruments in ELMAT & SKLEAC
Biography:
Li Niu has completed his PhD at the age of 30 years from Changchun Institute of Applied Chemistry and postdoctoral studies from the Åbo Akademi Unversity. He is the Director of Engineering Laboratory for Modern Analytical Techniques, CIAC and CAS. He has published more than 170 papers in reputed journals and has been serving as Editorial Board Members in several journals.
Abstract:
Fundamental electrochemical investigations in combination whatever with materials, environment, life science, and other disciplines, only those 3 possible & practical applications for the human being are available at the moment, such as new methods, devices and instruments. As known, electrochemistry technique is an important member in the whole family of the instrumental analysis. Especially, in coupling with other techniques, we can know much information about the interfacial interaction, structural features, reaction process, mass transfer, etc. during electrochemical running. Unfortunately, imported instruments & equipment occupies the leading position in China within the past decades, electrochemistry system is also still the world of imported products in China, such as Princeton, CHI, BAS, Gamry, Biologic, etc. Besides those electrochemical instruments, some typical and daily-used electrochemical sensors, such as blood glucose analysis, industrial control gas sensors, heavy metal ion monitoring, blood gas analysis, met the same problem in China. With the great increase of human industrial production, water quality analysis is becoming more and more necessary. A few typical electrochemical devices and methods for water monitoring, such as DO, COD, heavy metals, etc. have been developed successfully. In addition, various methods and sensors for bioanalysis & food analysis have been explored too. Furthermore, a series of electrochemical instruments has been completed, which ranged from basic models to advanced, from potentiostat to bipotentiostat, even to multichannel, from integrated spectrometers to electrochemical imaging & etching, etc. Those developed instruments have been widely used in many institutes & universities in China.
Pawanpreet Singh
University Institute of Pharmaceutical Sciences, India
Title: Solid state analytical techniques to characterize cocrystals: Ciprofloxacin–hippuric acid co-crystal
Biography:
Pawanpreet Singh has completed his Masters in Pharmacy from Punjab Technical University at the age of 23 and is presently doing PhD from University Institute of Pharmaceutical Sciences, Punjab University, India. He is doing his research work on the topics like crystal engineering, polymorphism and crystal structure prediction.
Abstract:
Co-crystals are crystalline single phase materials composed of two or more different molecular and/or ionic compounds generally in a stoichiometric ratio which are neither solvates nor simple salts. They are of interest to both academic research as well as industry as they represent the opportunity to access new solid forms with modified physical properties including dissolution rate, physical stability and altered pharmacokinetic profile of the API subjected for co-crystallization. The present work represents Ciprofloxacin-Hippuric acid cocrystal which was prepared by solvent assisted grinding method. It was primarily characterized by FTIR showing shift in –COOH stretch at 3264.1 cm-1 in cocrystal as compared to 3405.2 and 3343.9 cm-1 in drug and coformer respectively. The C-N stretch also shifted from 1380 cm-1 to 1385.2 cm-1. The cocrystal was then confirmed by Differential scanning calorimetry showing single melting endothermic transition at 218.9oC irrespective of both components showing formation of new stable phase. XRPD pattern of cocrystal shows disappearance of two major peaks at 14.7 and 24.9o 2θ while another major peak shows drastic shift from 25.5 to 19.5o 2θ. Fortunately, the development of XRPD equipment and structure solving algorithms makes it possible to extract the crystal structure from the XRPD pattern. Recently, peers introduced a protocol to confirm the refined result using combination of XRPD and solid state NMR data. The NMR chemical shift values (1H, 13C, 15N) were also computed to validate the refined cocrystal structure.
Tan Guan Huat
University of Malaya, Malaysia
Title: Electrochemical preparation and characterization of gold nanoparticles graphite electrode: Application to myricetin antioxidant analysis
Biography:
Guan Huat Tan is Professor in Analytical Chemistry at the Department of Chemistry, University of Malaya. He started his teaching career at the University of Malaya in 1978 after completing his PhD in Analytical Chemistry from Duke University. He has supervised eight PhD and six MSc students and many thesis projects on environmental analysis of organic chemical pollutants in Malaysian waterways and pesticides in fruits and vegetables by using techniques such as GC, GC-MS, HPLC and LC-MS. He is currently supervising five PhD and one MSc student for their theses. He is also currently doing studies on developing microextraction techniques coupled to GCMS and LCMS for analysis of pesticide residues in food matrices such fruits and vegetables. He has presented and published many papers on the monitoring of priority environmental organic pollutants at various international and local symposiums and conferences as a result of his research findings.
Abstract:
Graphite has been an attractive material for electrochemical analysis due to it attributes in terms of its physical, chemical inertness and also because of its low cost, abundantly available, reusable and can be obtained in various forms such as rod, sheet, and flexible sheets. However its electrochemical properties are often a drawback when compared to other solid metal electrode, but this can be improved by surface modification of the graphite with noble metal nanoparticles. In recent years, antioxidant analysis by electrochemical techniques has been the focus of many studies; this is because the technique is sensitive, rapid and possible for field analysis. These advantages had overcome the problem of anti-oxidant analysis, which are sensitive to environment oxidation and therefore a fast analysis procedure is always preferable. In this present paper, we have carried out the electrochemical improvement of a recycled graphite electrode through an optimized procedure by electro-deposition of the gold nanoparticles on the graphite surface. An improvement on the electrochemical properties of the graphite by enhancement of its effective surface area, heterogeneous electron transfer rate, over potential and also the sensitivity has been observed. Application of the fabricated gold nanoparticles graphite electrode was also successfully optimized for the qualitative and quantitative analysis of myricetin.
- Track:3 Bio analytical Methodology
Track 4: Analytical Techniques in Pharmacogenomics
Track 5: NMR and Analysis of Small Organic Molecules
Session Introduction
Khondaker Miraz Rahman
Transcriptogen Limited, UK
Title: Targeting transcription factors with DNA interactive small molecules
Biography:
Khondaker Miraz Rahman graduated as a pharmacist from the Faculty of Pharmacy of University of Dhaka, Bangladesh in 1996. He worked for 3 years as a research and development pharmacist at SK&F Pharmaceutical before moving to academia in April 2001 and joined the Pharmacy department of University of Asia Pacific as a Lecturer. In October 2003, he was appointed as a Lecturer in pharmaceutical chemistry at Dhaka University and was promoted to Assistant Professor in June 2005. He completed his PhD research at the London School of Pharmacy (now UCL School of Pharmacy) under the supervision of Professor David Thurston. He joined the CRUK Protein-Protein Interaction Research Group as a CRUK Research Fellow in July 2009. He was appointed as a Lecturer in Medicinal Chemistry at King’s College in May 2012.
Abstract:
Transcription factors are regulatory macromolecules that induce profound and sustained effects in cells by interacting with, and modulating the expression of genes responsible for critical cellular processes. Interaction of a small molecule with the consensus DNA sequence can prevent a transcription factor from recognizing its cognate sequence, thereby preventing expression of genes associated with the transcription factor. Transcription factor inhibition is an exciting new area of drug discovery, and is considered by some experts to represent the “next wave” of cancer therapeutics following the kinase inhibitors (which have now reached maturity) and the antibody-based approaches which are now in the ascendancy. There are few approved drugs at present that work by selectively inhibiting transcription factors, and so there is significant clinical and commercial potential. From a scientific/clinical perspective, this approach has the advantage that a selective and potent inhibitor would act at the ultimate signalling point of gene expression (i.e., the promoter region of a gene) thus directly modulating the expression of genes carrying the cognate DNA recognition site of the targeted transcription factor. Furthermore, there are sub-families of transcription factors (e.g., STAT1 and STAT3), and it may prove possible to target these independently, thus achieving fine-control over transcription. This is an important distinction from the kinase inhibitors that also modulate gene expression, but have multi-pathway downstream targets and are rarely highly selective, as their kinase target will usually control a range of transcription factors. The talk will explore innovative DNA targeting therapeutic approaches to develop low molecular weight “druggable” molecules that can be targeted to unique transcription factor recognition sites in the human genome. Various mechanisms can be used including the inhibition of protein-protein interactions (PPIs) and protein-DNA interaction (PDIs). A number of duplex-DNA and promoter G- Quadruplex-binding agents are being developed to target the PDI interaction, and some examples will be presented
Aly Moussa
Anses Lyon Laboratory, France
Title: The biological activity of streptomycin and related molecules are associated with the presence of 2 functional guanidine groups
Biography:
Aly Moussa obtained his BVSc from Cairo University, Egypt; Dr. Vet. Med. From Justus Liebig university, Germany and PhD from Claude Bernard University, France. He worked 4 years at IFFA-Mérieux Laboratory; Lyon- France, for 20 years was the chief of virology service at the French Bovine Pathology laboratory. Then for 8 years he was concerned at the national agency for sanitary security of aliments with research on the pathogenic prion proteins. He has published many papers in the fields of Virology and Transmissible Spongiform Encephalopathy’s.
Abstract:
The presence of at least 2 functional guanidine groups within a non-polymeric hydrophilic molecular system was suspected to be the chemical structure of streptomycin implicated in the interaction with proteins. To prove this hypothesis, several chemicals possessing two guanidine groups as streptomycin (dihydrostreptomycin, bis-3-aminoproylamine, guanidine hydrochloride, triethylene tetra mine and spermine tetra-hydrochloride) were tested for evaluating their interaction with the pathogenic prion protein (PrPsc). All molecules sharing common chemical function with streptomycin reproduced aggregation and precipitation of the prion protein. The interaction of streptomycin with proteins is optimum at alkaline PH and takes place through hydrogen bond transfer between the 2 guanidine groups on streptomycin and the amino-acids of one or several prion peptides ruling the possibility of a Schiff-base reaction. Streptomycin had proved valuable for earlier and higher immunological detection of prions in clinical samples due to protein aggregation as well as to a better attachment of antibodies to their epitopes through electric charge transfer on the protein surface. These changes of the surface electrostatic charges induced by streptomycin affect also the prion stability leading to a reduced infectivity.
Joshua M Hicks
Catalent Pharma Solutions, USA
Title: NMR quantification in the verification of compounds: Simple to complex mixtures
Biography:
Joshua M Hicks has completed his PhD from Oregon State University in 2005 from the department of Biochemistry & Biophysics, and a Postdoctoral tenure at Stockholm University. He is a Lead Scientist in the Structural group at Catalent, a premier drug development, delivery and supply partner organization for drugs, biologics, and consumer health products. He has been highly active in the design, development and implementation of NMR applications in pharmaceutical applications for over 9 years.
Abstract:
Quantification is one of the most important pieces of information when using compounds in testing. It is important that the correct concentration is used when measuring within applications such as monitoring of reactions, proteinligand binding for the measurement of binding constants, dosage, fragment based screening, and monitoring of branching rates of polymers, among others. Such rate limiting steps are self evident in compound library screening where one must verify concentration in a high throughput means. With the improvements in software and NMR instrumentation, it is possible to have on the fly verification where concentration can be a key part of the verification process. Since the submitter has knowledge of the (suggested) structure, a known submission mass and a volume, the calculations for integral accounting and assignment can be output. Concentration is a key aspect because deviations are indicative of compounds purity from other compounds as well as impurities not directly observable by NMR such as salts. Additionally the solubility of the compound and stability are observed due to large changes in calculated versus measured concentration. All important aspects when validating a regular method for development/validation of definitive verification of a compound or mixture of compounds.
Mei-Hwei Tseng
University of Taipei, Taiwan
Title: Determination of activated methyl cycle metabolites and glutathione in cabbage upon sulfate deprivation and copper toxicity
Biography:
Mei-Hwei Tseng had completed her PhD from National Taiwan University and Postdoctoral studies at the Laboratory of Plant Physiology, University of Groningen, The Netherlands.
Abstract:
The activated methyl cycle is a central metabolic pathway used to generate (and recycle) several important sulfur-containing metabolites including methionine, S-adenosylmethionine, S-adenosylhomocysteine and homocysteine and enable methylation. Glutathione is a ubiquitous intracellular peptide with diverse functions that include antioxidant defence and detoxification of toxic metals and xenobiotics. Glutathione is present in plant tissue in the thiol-reduced (GSH) and disulfide-oxidized (GSSG) form. We had developed a precise and sensitive method for the simultaneous measurement of several sulfur metabolites based on liquid chromatography coupled with tandem mass spectrometry and 34S-metabolic labelling of sulfur-containing metabolites in Arabidopsis thaliana seedlings. Sulfur metabolites were extracted from freeze-dried roots and shoots of cabbage (Brassica pekinensis) after 14 and 21 days of plant cultivation with an aerated 25% Hoagland nutrient solution. The impact of sulfate deficiency and copper toxicity (10 µM) on the levels of activated methyl cycle metabolites and glutathione in cabbage were determined by our method, which is based on stable dilution-based liquid chromatography-electrospray ionization-tandem mass spectrometry. The levels of these metabolites only declined during the initial period of copper toxicity and most of them were further restored, while all the levels of these metabolites except that of S-adenosylmethionine in the shoot declined during sulfate deficiency, resulting in significantly higher ratios of S-adenosylmethionine/S-adenosylhomocysteine and GSH/GSSG in the shoot.
Vitor H Pomin
Federal University of Rio de Janeiro, Brazil
Title: Advances in structural biology of glycosaminoglycans by 15N-NMR spectroscopy
Biography:
Vitor H Pomin (MS, PhD) is an Assistant Professor of Biological Chemistry, Biochemistry, Glycobiology, and NMR Spectroscopy at Institute of Medical Biochemistry Leopoldo de Meis, Federal University of Rio de Janeiro, Brazil, since May 2011. He pursued his undergraduate studies in Biological Sciences and graduate studies in Biological Chemistry at the same university. He received his Diplomas of Licentiate, MS, and PhD in 2003, 2005 and 2008, respectively. His MS and PhD were supervised by Prof. Paulo A S Mourão. After this period, he pursued a Post-doctorate experience at the Complex Carbohydrate Research Center, University of Georgia, United States, until April 2011, under the supervision of the Eminent Scholar of NMR spectroscopy Prof. James H. Prestegard. He has over 40 published articles in high-impact peer-reviewed journals, 11 book chapters, besides being editor of 6 academic/scientific books. He serves as an Editorial Member and frequent reviewer of many internationally recognized journals like Biopolymers, Carbohydrate Polymers, Biochimica et Biophysica Acta-General Subjects, Marine Drugs, Phytochemistry, the Journal of Biological Chemistry, Glycoconjugate Journals, ACS Biochemistry, Current Protein & Peptide Science, and others. He conducts research on glycobiology (especially sulfated polysaccharides), structural (glyco) biology and NMR spectroscopy.
Abstract:
With the recent developments to enhance sensitivity in solution NMR spectroscopy, such as the advent and spread of high-magnet fields worldwide, cryoprobe technology, isotopic labeling techniques, and new combinations of 2D pulse sequences, a new direction in structural analysis of glycosaminoglycans (GAGs) by NMR spectroscopy has emerged. The current scope is the one more dedicated to the less sensitive amide 15N isotope of hexosamines rather than the commonly used anomeric and ring 1H- or 13C-resonances of uronic acids and hexosamines. Given that GAG types are basically classified upon their composing hexosamine types together with the variations of their sulfation patterns, 15N-related NMR studies on native GAGs, oligosaccharides or the different composing amino sugars have proved to be quite informative in both structural or dynamic point-of views, despite the low number of the resultant NMR signals. This in turn reduces significantly chemical shift degeneracy as at the same time facilitates spin/structural assignments. This section is dedicated to cover the principal contributions made so far by solution 15N-NMR spectroscopy toward the progress of the structural biology of GAGs in the current glycomic age.
MarÃa José Ruiz-Ãngel
University of Valencia, Spain
Title: Comparison of the performance of amines and ionic liquids as additives in RPLC for the analysis of basic compounds
Biography:
María José Ruiz-Ángel obtained her PhD from the University of Valencia (Spain) in 2003. In 2004‒2006, she was granted with a Post-doctorate fellowship in the Laboratoire des Sciences Analytiques at the University Claude Bernard in Lyon (France). In 2007, she was awarded with a Ramón y Cajal research position in the Department of Analytical Chemistry at the University of Valencia, where she is Professor since March 2012. She has written over 50 research articles, most focusing on secondary equilibria using surfactants and ionic liquids, fundamental studies in HPLC and development of analytical methods for pharmaceutical and clinical samples.
Abstract:
The residual silanols, present in silica-based stationary phases used in reversed-phase liquid chromatography, are responsible for additional ion-exchange interactions with positively charged solutes that result in undesirable broad and tailed peaks. In order to enhance the chromatographic performance of these compounds, amines of different nature have been traditionally added to the mobile phase. These cationic reagents decrease the silanol activity by covering or blocking the silanol sites, which yields symmetrical peaks. The number of available amines as potential additives is high, but recently, the possibilities have even expanded with the introduction of ionic liquids (ILs) as a powerful alternative. In consequence, the offer is now so wide that the selection of the most suitable blocking silanol agent is not easy, being possible that among different solutions the best is not always chosen. ILs seems to be the fashionable option, which has somehow relegated the use of classical amines, or extended wrong ideas about the supposed ineffective results of amines as silanol suppressors. Several reasons for these assumptions can be addressed: The reduced number of amines that are compared with ILs, insufficient understanding of the mechanisms of retention, and an incorrect interpretation of the silanol suppressing potency, which is exclusively evaluated based on the retention behavior. In this work, a comprehensive study of the silanol suppression effect given by two amines and a group of ILs for the analysis of a set of basic β-blockers is carried out. The results are analyzed considering retention and peak shape, which allows elucidating the protection mechanism of both types of additives.
- Track 6: Advances in Chromatography and Mass Spectrometry
Session Introduction
Mohammed Farrag El-Behairy
National Research Centre, Egypt
Title: Enantiomeric separation of biologically active 2-Pyrazolines on Lux Cellulose-2 and Lux Amylose-2: A comparative study
Biography:
Mohammed F. El-Behairy has completed his BSc of pharmacy on 2003 with grade excellent honors and PhD at the age of 29 years from Faculty of Pharmacy, Cairo University as joint supervision between NRC, Egypt and NTNU, Norway and postdoctoral studies from NRC, Egypt and IECB, France. He has awarded best PhD prize in the National research Centre of Egypt (Pharmaceutical Sciences) on 2012. He has published more than 13 papers in reputed journals and has been serving as a reviewer board member of repute.
Abstract:
The enantioselective resolution of a set of biologically active 3-(benzo[d][1,3]dioxol-5-yl)-5-tert-butyl-4,5-dihydropyrazole derivatives (1-21) is demonstrated. Two chiral stationary phases based on the chloromethylphenylcarbamate derivatives of amylose or cellulose: namely Lux Cellulose-2 and Lux Amylose-2, were evaluated for the chiral separation of theses 2-pyrazolines using enantioselective high performance liquid chromatography (HPLC). Different mobile phases and separation modes have been investigated supported by the versatility of Lux columns. Baseline separations of all investigated compounds have been achieved using different mobile phases such as (pure Methanol, Acetonitrile, or Ethanol, or Mixtures of Methanol/Acetonitrile, Acetonitrile/Ethanol, Hexane/Ethanol). In general, Cellulose-2 column showed better enantio-separation for most of the compounds represented by high resolution in shorter time. For example compound 4 (R = H) showed Rs = 20 and run time 36 min when using Acetonitrile 100% on lux cellulose-2 while using Methanol/Acetonitrile 1/1 v/v showed Rs = 8 and run time up to 6.0 min (Rs = 1.40 for amylose-2).
Joon Myong Song
Seoul National University, South Korea
Title: Hyper-multicolor high-content cellular assay based on quantum dot nanoprobe
Biography:
Joon Myong Song received his PhD in 1997 at Kyushu University, in Japan. He worked as a Postdoctoral Research Fellow from 1998 to 2004 at Iowa State University, Brookhaven National Laboratory, and Oak Ridge National Laboratory in United States. At present, he is a Professor and Head of Department of Pharmacy at College of Pharmacy School, Seoul National University in South Korea. His research area includes multifunctional nanoparticle for diagnosis and therapy and high-content cell-based drug screening and diagnosis using hyper-multicolor cellular imaging. He has published 84 peer reviewed papers in the top journals, 7 book chapters, and 10 patents.
Abstract:
High-Content Cell-Based Assay (HCA) has attracted great attention due to its ability to be used in the drug discovery-driven research and development required to understand the functions of genes and gene products at the level of the cell. HCA simultaneously measures multiple biomarkers in a single cell with multiplexing fluorescent probes. The complex intracellular responses involved in drug-induced efficacy or cytotoxicity can be observed in organ-specific cells by HCA. Application of HCA to organ-specific cell models provides deeper biological information suitable for better decisions on progressing compounds. Early safety evaluation by HCA reveals the complex cellular responses triggered by potentially harmful molecules in the cells of target organs. Gaining a deep understanding of the mechanisms underlying these cellular toxicological responses is valuable before a series of lead compounds are progressed to time-consuming and expensive animal tests. Despite HCA’s capability, it is not common to simultaneously observe many biomarkers in an intact cell. This is because HCA measurement is dependent on the use of probing materials. Concurrent monitoring of multiple biomarkers is practically limited due to the spectral overlap among probing materials having broad absorption and emission spectrums. Quantum dot-based HCA is capable of supplying cellular imaging at particular wavelengths and each wavelength can be scanned rapidly. This cellular imaging is very advantageous in that it can select particular wavelengths that do not overlap among the probing materials and concurrently monitor a large number of drug targets or biomarkers.
Toshinori Yamamoto
RaQualia Pharma Inc., Japan
Title: Use of “Omics†technologies for mechanistic understandings of toxicological events
Biography:
Toshinori Yamamoto, PhD is currently VP and Head of Preclinical Research, RaQualia Pharma, Japan. He experienced broad range of Pharmaceutical R&D covering Discovery through Regulatory Filing, wherein mainly focusing on DMPK, toxicology and clinical development for more than 20 years. Before joining RaQualia in 2008, he worked at Chugai Pharmaceuticals and Pfizer, Japan. He was a visiting researcher at the University of Arizona and the University of Michigan. He was awarded the 2010 Incentive Award from the Japanese Society of Toxicology (JSOT), and the JSOT Tanabe Award in 2006, 2007 and 2008.
Abstract:
Drug toxicity observed in pre-/non-clinical animal studies sometimes leads to discontinuation of drug candidates. Understanding the phenomena or backgrounds surrounding toxicological events occurred must be a key element for attrition improvement in research and development (R&D) of new chemical entities (NCEs), often requiring mechanistic investigations to understand toxicological mechanism of actions and then to make “Go” or “No go” decisions. In the post-genomic era, a battery of “Omics” technologies was introduced and has been rapidly increasing utilization, among which is the prefix of “toxico-” added to each omics technology in toxicology field. Especially, metabolomics in toxicology, which is so-called “toxicometabolomics”, has been widely implemented in this area. The objectives are to identify and characterize the metabolites, both endogenous and exogenous, which are the end products of cellular metabolism and drug metabolism, respectively. Moreover, toxicometabolomics enables to capture the phenotypic changes in the events, which are generated by enzymatic proteins as resultants of gene expression, at the molecular level; therefore, smooth translation of the findings can be made into the clinic. The presentation in this session will review the usefulness of toxicometabolomics technologies which are generally nuclear magnetic resonance (NMR)-based and mass spectrometry (MS)-based, and other toxic-Omics technologies. Furthermore, today, newly introduced technology, MS imaging (MSI) is considered applicable in the toxicology field, hence its toxicological usability will be also reviewed.
Biography:
Alla Kloss has a Masters Degree in Chemical Engineering from The Institute of Technology (St. Petersburg Russia), has also completed her PhD in Physical/Analytical Chemistry from University of California, Davis and Post Doctoral studies at the University of Illinois at Urbana-Champaign. She is a Scientific Director at Analytical Research and Development Department in LGCR, Sanofi where she leads a biomarker discovery group.
Abstract:
As translational science approach to drug discovery and development is being broadly implemented in pharmaceutical industry we are increasingly relying on metabolic biomarkers in all phases of the process, including target credentialing, proof of concept, establishment of biomarkers based screens, in vivo studies, clinical studies and establishment of biomarker based diagnostics. In cases when biomarkers are not known, metabolomics platform becomes an essential tool for holistic and unbiased interrogation of thousands of metabolites and identification of metabolic biomarkers. Identification of biomarkers is based on global profiling using chromatographic separation coupled with high resolution accurate mass spectrometric detection (HRAM). Processing of the data is carried out using sophisticated peak picking software, statistical packages and working with data bases. Since there is no single “of the shelf” product enabling to carry out all the data processing steps for metabolomics, this is especially challenging. Exact molecular mass information available in public data bases typically allows only for tentative identification of the biomarkers and often results in multiple options which have to be evaluated in additional experiments. This is both expensive and time consuming. In this presentation we will describe analytical platform for metabolomics established at AR&D / LGCR in Sanofi as a result of multiple applications and cross-departmental collaborations. We will discuss considerations for development of methodology for sample preparation and analysis using LC-HRAM, as well as approach for construction of an in-house metabolomics data base and demonstrate our data processing approach based on trends and correlation analysis allowing for identification of phenotype-linked biomarkers with much reduced effort.
Biography:
Eduard Rogatsky is a senior faculty member at Albert Einstein College of Medicine (Bronx, NY). He is also the Director of mass spectrometry at the Biomarker Analytical Resource Core as part of the Harold and Muriel Block Institute for Clinical and Translational Research at Albert Einstein and Montefiore medical centers. He has been in the field of chromatography for more than 20 years and has 14 years of experience in clinical mass spectrometry. Currently, he serves as the Editor-in-Chief for the Journal of Chromatography and Separation Techniques (OMICS Publishing Group). During the last 10 years (from 2005), he has published over 30 scientific papers in per-reviewed journals (mostly as the first author) and has presented over 50 posters and lectures. Overall, he has made more than a hundred scientific presentations and publications. He completed his MSc in Physical Chemistry at Belarus State University (former USSR) in 1990. He completed his PhD in Bioanalytical Chemistry (Bar-Ilan University, Israel) in 1998. At the end of 1999, he started his Post-doctorate at Albert Einstein College of Medicine and became a faculty member is 2001. Presently, he holds the title of Research Associate Professor of Medicine.
Abstract:
Different chromatographic and mass spectrometry instrumentations are used in modern bioanalytical laboratories. We noticed that transfer of existent liquid chromatography-mass spectrometry method from one LC/MS system to another is not a simple task. In addition, even adoption of an already published and well validated method is not as straightforward as one would expect. On real world examples, we will discuss instrument-dependent, sample-dependent, and method-dependent variability and implications. To solve these issues, it requires detailed understanding of the method, hardware, assay parameters and analyte nature.
Evgeny Nikolaev
Institute for Energy Problems of Chemical Physics of RAS, Russia Moscow Institute of Physics and Technology (Fiztech), Russia Skolkovo Institute of Science and Technology (Skoltech), Russia
Title: Analytical possibilities of ultra-high resolution mass spectrometry
Biography:
Evgeny (Eugene) N Nikolaev has completed his PhD from Moscow Institute of Physics and Technology and habilitation (Dr.Sc.) from the Institute for Energy Problems of Chemical Physics. He is the Head of three research laboratories in Russian Academy of Sciences and Professor of Fiztech and Skoltech. He has published more than 223 papers in reputed journals and has more than 50 patents. He is the Editorial Board Member of Journal of Mass Spectrometry, European Journal of Mass Spectrometry and Rapid Communications in Mass Spectrometry. He is the Organizer of the 8th European Conference on FT ICR mass spectrometry and the First International Conference on Innovations in Mass Spectrometry Instrumentation (2013).
Abstract:
Mass spectrometry is widely used in analytical and bio analytical chemistry. Mass spectrometry of ion cyclotron resonance with Fourier Transform demonstrated the highest resolving power among other types of mass spectrometers since its opening in 1974 by Marshall and Comisarow. Recently, resolving power of FT ICR MS jumped by almost order of magnitude with introduction of the new type of FT ICR ion trap called dynamically harmonized ICR cell. Implementation of this cell on FT ICR instruments with moderate magnetic fields (5-7) Tesla permits to reach about 10 million resolving power on ion masses corresponding to mass to charge ratio m/z close to 1000 Da. With such resolving power, it is possible to resolve isotopic fine structure of molecular ions and by this to determine their atomic composition. Examples of isotopic fine structures resolution and application to analyses of crude oil, humid substances and extraterrestrial organic will be given. The initial implementation of the simple, reliable and reproducible in-ESI source H/D exchange approach coupled to ultrahigh resolution FTICR MS was performed for enumeration of labile hydrogens in individual molecules on the cluster of phosphorous acids, natural organic matter, oligosaccharides, peptides and proteins of different mass from 1 kDa to 66 kDa.
Jun Aoki
Osaka University, Japan
Title: Development of new stigmatic imaging mass spectrometer and its application to metal cation distribution in fish
Biography:
Jun Aoki has completed his PhD from Kyoto University. He is the Assistant Professor of Osaka University. His field of expertise is imaging mass spectrometry. He prized the Mass Spectrometry of Japan Research Award in 2012.
Abstract:
Measurement methods of spatial distribution of molecules such as proteins and drugs at cellular-scale are required in many fields. Recently, scanning type imaging mass spectrometry (IMS) with matrix-assisted laser desorption/ionization (MALDI) is intensively used for biomolecular analysis. However, the spatial resolution of scanning MALDI-IMS is limited by to about 10 - 100 μm and inadequate for cellular-scale observation. Therefore, we are developing a stigmatic MALDI imaging mass spectrometer for higher spatial resolution. The experimental apparatus consists of a MALDI ion source, a multi-turn time-of-flight mass spectrometer (MULTUM) and a time and position sensitive delay line detector. Ion distributions at the sample plate are magnified and projected with the ion optical lens system onto the detector. The ion optical system of MULTUM satisfies the perfect spatial and temporal focusing condition, so that the spatial distributions of ions conserved after circulation. Our evaluation experiment demonstrated that both the spatial resolution of 1 micro-meter and the mass resolving power of 10000 were simultaneously achieved. We applied this new apparatus to several practical applications, for example observation of the distribution of accumulated metal cations in fish. We used medaka (Oryzias latipes) as samples for observing the bioaccumulation of Sr and Cs. Medaka were raised for two weeks in water containing 0.001 mol/L SrCl2 and 0.05 mol/L CsI. Distributions of Cs and Sr in a sliced section of medaka are obtained with a micrometer-scale spatial resolution.
Yahdiana Harahap
Universities Indonesia, Indonesia
Title: The effect of anticoagulant types in analyzing levofloxacin in human plasma by high performance liquid chromatography-photodiode array
Biography:
Yahdiana Harahap received her education in Indonesia with BSc degree (1987) at Department of Pharmacy Faculty of Mathematics and Natural Sciences University of Indonesia. She obtained her MS (1994) and PhD (2003) in Pharmaceutical Chemistry at Department of Pharmacy Faculty of Mathematics and Natural Sciences Institute Technology Bandung. She then worked as Head of Public Service Center at Department of Pharmacy, University of Indonesia. Now she is a part of Bioavailability and Bioequivalence Laboratory, Faculty of Pharmacy, Universities Indonesia and member of BA/BE working group Indonesia, also as the Bioequivalence expert at National Agency of Drug and Food Control Republic of Indonesia.
Abstract:
Levofloxacin has low concentration in plasma, thus it requires sensitive and selective analysis method. Plasma drug analysis often uses many kinds of anticoagulant to obtain plasma as analytical matrix. Citrate, heparin, and ethylenediaminetetraacetic acid (EDTA) are anticoagulant commonly used in analyzing drug in human plasma. This study was focused on analyzing levofloxacin in human plasma with three types of anticoagulants. The analysis was performed using High Performance Liquid Chromatography (HPLC) – photodiode array with Column C18 SunfireTM (250 x 4.6mm), 5ïm; temperature of 45ï¯C, mobile phase consist of 0.5% triethylamine pH 3.0 -acetonitrile (88:12 v/v); flow rate of 1.25 mL/minute, and ciprofloxacin HCl as internal standard. The method was linear at concentration range of 50.0 – 10.000.0 ng/mL with r>0.9994. Accuracy and precision for citrate, heparin, and EDTA plasma fulfilled the acceptance criteria of both intra-day and inter-day. There was no significant difference for stability and recovery of levofloxacin in citrate, heparin, and EDTA plasma (p>0.05; ANOVA), but it showed significant difference for peak area ratio (p>0.05), between citrate-EDTA plasma and heparin-EDTA plasma for low concentration and between citrate-heparin plasma and citrate-EDTA plasma for mid and high concentration. On blank chromatogram EDTA plasma, there was interference on retention time of less than 8 minutes, while on citrate and heparin plasma there was no interference. The method can be applied for bioequivalence study using the three anticoagulants that are equally good.
Zsuzsanna Kuklenyik
Centers for Disease Control and Prevention, USA
Title: Identification and quantification of proteins participating in apoA-1 and apoB-100 mediated cholesterol transport by field-flow fractionation and LC-MS/MS analysis
Biography:
Zsuzsanna Kuklenyik has completed her master degree in chemical engineering at Technical University of Budapest, and her PhD degree at Emory University of Atlanta Georgia, where she also conducted Postdoctoral studies. Currently she is a Senior Research Scientist in the Biological Mass Spectrometry Laboratory at the Centers for Disease Control and Prevention in Atlanta. She has published more than 40 papers in reputed journals on wide range of applications of hyphenated chromatographic techniques and mass spectrometry, such as biomonitoring of environmental chemicals, analysis of pre-exposure prophylactic drugs against HIV, biological toxins, and more recently, lipoproteins.
Abstract:
Lipid homeostasis in vivo is mediated by several lipoprotein sub-species, commonly classified as high density and low density lipoproteins (HDL and LDL). The two common apolipoproteins, ApoA-1 and ApoB-100 are associated with numerous exchangeable lipids and proteins in various compositions. Because of their number and complexity, the analysis of lipoprotein sub-species in plasma by bottom-up proteomics approaches requires pre-analytical physical fractionation. Asymmetric flow-field flow fraction (AF4) is a gentle separation technique based on hydrodynamic size. AF4 allows size separation of intact lipoprotein sub-species and fraction collection. LC-MS/MS analysis of the individual fractions can provide unique concentration versus hydrodynamic size profiles for each protein constituent. Deconvolution of the concentration versus size profiles of ApoA-1 and ApoB-100 allows quantitative deduction of the particle number of lipoprotein sub-species. The probability of associations of proteins with ApoA-1 and ApoB-100 containing sub-species was evaluated based on size profile overlap with those of Apo-A-1 or ApoB-100 sub-species, size derived maximum possible total molecular weight, and protein-ApoA-1 or protein-ApoB-100 concentration correlations measured in multiple serum samples with wide range of cholesterol and triglyceride levels. Based on these criteria proteins can be included or excluded as participants of ApoA-1 and ApoB-100 mediated lipid metabolism pathways.
- Track 7: Analytical Techniques in Immuno Chemistry
Track 8: Environmental Analytical Aspects
Session Introduction
Cheng-Chuan Su
Buddhist Dalin Tzu Chi Hospital, Taiwan
Title: Uremic effect on immunofluorescence and enzyme-linked immunosorbent assays for detection of human herpes virus type 8 antibodies
Biography:
Cheng-Chuan Su completed residency training in Anatomic Pathology at the age of 31 years and in Clinical Pathology two years later; and obtained the Master degree from the Institute of Biomedical Engineering, National Cheng Kung University, Taiwan when he was 32 years old. At present, he is the attending physician of the Departments of Anatomic Pathology and Clinical Pathology, Buddhist Dalin Tzu Chi Hospital, and the Associate Professor of the Departments of Laboratory Medicine and Pathology, Tzu Chi University, Taiwan. He has published more than 40 papers in reputed journals.
Abstract:
Human herpes virus type 8 (HHV-8) is the etiologic agent of Kaposi’s sarcoma (KS). The incidence of KS in renal transplant patients is much higher than in healthy controls. The risk is even higher among recipients seropositive for HHV-8 before transplantation. Patients with end-stage renal disease (ESRD) are immuno compromised and are candidates for renal transplantation, but HHV-8 seroprevalence in ESRD patients was not well documented. Our previous study showed that seropositivity and titers for HHV-8 antibodies with immunofluorescence assay (IFA) as well as seropositivity with enzyme-linked immunosorbent assay (ELISA) were significantly greater in ESRD patients than in healthy controls (P = 0.006, 0.001 and 0.003, respectively). Patients with a history of taking herbal medicine had significantly greater ELISA positivity than those without such a history (P=0.004). ELISA positives, particularly patients, had much higher IFA antibody titers than ELISA negatives (P<0.0001). Seropositivity in ESRD patients was not related to lymphopaenia, monocytosis, dialysis duration, or a history of transfusion. Two diabetic ESRD patients were positive for HHV-8 DNA. However, other studies indicated that ESRD patients and healthy controls had similar HHV-8 seroprevalence. Hence, we further investigated whether this discrepancy is due to the effect of uremic status. The results showed that HHV-8 seropositivities based on IFA and ELISA, both before and after hemodialysis, were significantly greater in ESRD patients than in healthy controls (p<0.008 for all comparisons). The seropositivities and antibody titers of ESRD patients obtained with IFA were similar before and after hemodialysis. Seropositivities based on ELISA were identical before and after hemodialysis. The seropositivities obtained with the IFA markedly exceeded those with ELISA in each group of subjects (p<0.0001 for all comparisons).
Bonnie Tay-Jones Yen Ping
Malaysian Palm Oil Board, Malaysia
Title: Gas chromatography with flame ionization detection of 1,4-dioxane in palm-based fatty alcohol ethoxylates
Biography:
Bonnie Tay-Jones Yen Ping received her Master of Science degree (organic chemistry) from University Putra Malaysia, Serdang, Malaysia. She is currently a principal research scientist attached to Quality and Environment Assessment Unit, Advanced Oleochemical Technology Division, Malaysian Palm Oil Board. Presently, she is actively involved in research (method development work to detect toxic contaminants/by-products) for the local oleochemical producers. She was the main author of several publications in reputed journals on topics relating to gel permeation chromatography analyses for palm-based polyols, chemometrics, basic oleochemical compositional analyses and method development for detection of contaminants/by-products in palm-oil derived oleochemicals.
Abstract:
1, 4-Dioxane, a toxic by-product may be formed during the ethoxylation of fatty alcohol. A simple and rapid method using gas chromatography with flame ionization detector (GC-FID) was developed to detect-1, 4-dioxane in commercial palm-based fatty alcohol ethoxylate (FAEO). This method involved spiking of 1, 4-dioxane into FAEO samples, and directly injecting the spiked samples into GC-FID. The method was validated according to the ICH harmonized tripartite guideline. The calibration curves for 1, 4-dioxane showed good linearity with correlation coefficient of 0.9999. The accuracy of the method was indicated by recovery obtained for spiked 1, 4-dioxane samples at 5 levels of spiking, i.e. at 30, 60, 100, 200 and 500 μg/g, where recoveries were within 99 – 105% with relative standard deviation (RSD) of less than 4.0%. The RSD values of the intra-day and inter-day precision were less than 1.0%. The limit of detection and quantification was 10 μg/g and 30 ug/g, respectively. The identity of 1, 4-dioxane recovered from the palm-based fatty alcohol ethoxylate was confirmed by a GC-mass spectrometer detector.
Nuria De Diego
Palacký University & Institute of Experimental Botany ASCR, Czech Republic
Title: Implementation of plant hormone quantification in Arabidopsis using UHPLC-MS/MS
Biography:
Dr. Nuria De Diego is presently a junior researcher in Plant Physiology in the Department of Chemical Biology and Genetics of Centre of the Region Haná for Biotechnological and Agricultural Research, Palacky University (Czech Republic). She works under Dr. Karel Dolezal´s supervision, studying plant response against different growth conditions including biotic and abiotic stress, and mainly at metabolic level where phytohormones are the most relevant molecules. Dr. De Diego has more than 12 years research experience that has implemented with new academic achievements, including a Postgraduate in Biotechnology, an international Doctorate in Biological Sciences and articles in international impact journals.
Abstract:
Modern plant physiology has clarified many important processes involved in plant development and crop yield. Among them, the knowledge of the mechanisms implicated in plant response against ambient fluctuations allows us to identify interesting genotypes. These processes have been reported to be regulated by molecules known as plant hormones. Cytokinins, gibberellins, auxins and abscisic acid are some examples of them. They regulate physiological processes such as apical dominance, plant transpiration by stomata closure or rooting. Although plant hormones are related to these processes, nowadays more information is needed about their signalling and action mode in plants. In this regards, our research group is constantly working in the improvement of protocols to analyse the variations of these molecules in different plants tissues grown under varied conditions. The wide experience in the study of these plant growth regulators and the use of leading technologies based on ultra-high performance liquid chromatography–tandem mass spectrometry (UHPLC-MS/MS) have permitted us to increase the number of metabolites detected as well as simplification of purification protocol. In addition, the inclusion of micro-extraction and immuno-affinity techniques reduces the needed amount of sample per analysis. In addition, our group has an avant-garde high throughput phenotyping to continuously monitor the relative growth rate and to collect wide range of fluorescence parameters. The use of this facility permits us to combine and corroborate the relationship between the plant physiological traits and the variations in hormone pathways and to identify those metabolites related to these processes. As example, new information about the involvement between photosynthesis, carbon metabolism and changes of cytokinin levels in Arabidopsis was found.
László G Boros
Harbor-UCLA Medical Center, USA
Title: Targeted 13C metabolic tracer fate association study and regression analysis reveal diverse cellular phenotypes
Biography:
Dr. Boros holds a Doctor of Medicine (M.D.) degree from the Albert Szent-Györgyi School of Medicine of Szeged, Hungary, and a Doctor of Philosophy (Ph.D.) title from the Academic Senate of the University of California, Los Angeles Division, United States of America. Dr. Boros currently is a Professor of Pediatrics, Endocrinology and Metabolism at the UCLA School of Medicine and Chief Scientific Advisor of SiDMAP, LLC. Dr. Boros is the co-inventor of the stable isotope-based dynamic metabolic profiling (SIDMAP) technology.
Abstract:
Regression statistics in this targeted tracer fate association study (TTFAS) is shown to reveal associations among diverse phenotypic metabolic products in fumarate hydratase-deficient UOK kidney tumor cells with defective glutaminolysis, reductive carboxylation for lipogenic citrate production, as well as the Warburg effect, using the [1,2-13C2]-D-glucose tracer. These co-existing profiles were revealed in previous 13C-glutamine and 13C-glucose tracer experiments. Herein UOK cells show a close Warburg-type correlation between consumption of glucose for 13C-lactic acid production (R2>0.98; glucose to lactate). On the other hand, proliferation-related macromolecule 13C labeling, such as that of RNA-derived ribose and lignoceric acid, correlates with the glucose-derived internally cross-labeled 13C-glutamine fraction (R2>0.98; glutamate to lignocerate and RNA ribose). The TTFAS approach reliably re-produces results and conclusions obtained via multiple 13C-tracer metabolic flux analyses using a single metabolic tracer to trim down versatilities, cost and time, involved in multiple metabolic tracer studies using 13C and deuterium as labeling atoms for diverse products.
Hamid Hashemi-Moghaddam
Islamic Azad University, Iran
Title: Molecularly imprinted layer-coated silica nanoparticles toward highly selective separation of active glycine from urine sample
Biography:
Hamid Hashemi-Moghaddam has completed his PhD at the age of 25 years from Islamic Azad University. He has published more than 40 papers in reputed journals and is Managing Editor of Journal of Chemical Health Risks.
Abstract:
This work reports the preparation of glycine imprinted polymer layer-coated silica nanoparticles toward analysis of trace glycine in complicated matrices. To induce the selective occurrence of surface polymerization, the polymerizable double bonds were first grafted at the surface of silica nanoparticles by the silylation. Afterwards, the glycine templates were imprinted into the polymer-coating layer through the interaction with functional monomers. The programmed heating led to the formation of uniform glycine-imprinted polymer layer with controllable thickness, and further improved the reproducibility of rebinding capacity. After removal of templates, recognition sites of glycine were exposed in the polymer layers. As a result, the maximum rebinding capacity was achieved with the use of optimal grafting ratio. There was also evidence indicating that the glycine-imprinted polymer nanoparticles compared with non-imprinted polymer nanoparticles had a higher selectivity and affinity to glycine. Moreover, using the imprinted particles as dispersive solid phase extraction (DSPE) materials, the recoveries of glycine determined by UV-Vis spectrophotometry were 84.6% in the spiked urine sample. These results show the possibility that the highly selective separation and enrichment of trace glycine from urine sample can be achieved by the molecular imprinting modification at the surface of silica nanoparticles.
Wayne Grant Carter
University of Nottingham, England
Title: Enrichment strategies for capturing proteins altered by post-translational modifications
Biography:
Wayne Grant Carter received his Honors degree and PhD in Biochemistry from the University of Southampton, studying protein post-translational modification and molecular signalling cascades. He is currently a Group Leader in the School of Medicine, University of Nottingham, with research focused upon protein post-translational modification and molecular mechanisms of hepato- and neuro-toxicology.
Abstract:
The extensive repertoire of protein Post-Translational Modifications (PTMs) enables the cell to orchestrate functional interplay of protein biomolecules. Indeed, alterations and/or disruptions in protein PTMs can have profound effects on cellular fates. Thus, in order to understand biological processes, there is a need to characterize all PTMs and dissect their functional roles. Recently, the advent of sensitive mass spectrometry has facilitated the detection of post-translationally modified proteins; however, the extensive heterogeneity of PTMs is prohibitive to global Mass Spectrometry (MS) since it produces complex overlapping changes in peptide masses. To circumvent this MS limitation, selective enrichment strategies can facilitate detection and characterization of specific types of protein PTMs. A review of chromatography methods that considers their benefits and limitations for isolating post-translationally modified proteins will be undertaken. Once a protein PTM is detected and isolated, there is a need to consider the stoichiometry, half-life, and ultimately, functional consequence (s) of the PTM, and methods and results that investigate these processes will also be discussed.
Pradeep Shelke
P. Wadhwani College of Pharmacy, India
Title: Validated stability-indicating high performance liquid chromatographic assay method for the determination of Dabigatran Etexilate Mesylate
Biography:
P.G. SHELKE is an Assistant Professor in the department of pharmaceutical analysis at P. Wadhwani College of Pharmacy, Yavatmal
Abstract:
Dabigatran Etexilate Mesylate was subjected to different ICH recommended stress conditions. Degradation of drug occurs almost in all conditions (hydrolytic, oxidative and photolytic) while mild degradation was seen with thermal stress. A validated stability-indicating HPLC method was developed for the analysis of drug in presence of its degradation products. The stressed samples of drug were analyzed using Kinetex C-8 column (5µ, 250×4.6 mm) column using a mobile phase composed of methanol: Water, which was delivered initially in the ratio of 70:30 (v/v) for 1 min, then changed to 90:10 (v/v) for next 9 min and finally equilibrated back to initial composition 70:30 (v/v) from 11 to 20 min. The flow rate was maintained at 1.0 ml/min and detection was carried out at 230 nm using 996 PDA detector. The method was validated in terms of linearity, accuracy, precision specificity and selectivity
Biography:
Nesrine T Lamie is an Assistant Professor in the Department of Analytical Chemistry and Faculty of Pharmacy at Cairo University, Egypt
Abstract:
Four, accurate, precise, and sensitive spectrophotometric methods are developed for the simultaneous determination of a binary mixture containing amlodipine besylate (AM) and atenolol (AT) where AM is determined at its λmax 360 nm (0D), while atenolol can be determined by different methods. Method (A) is absorption factor (AFM). Method (B) is the new Ratio Difference method (RD) which measures the difference in amplitudes between 210 and 226 nm of ratio spectrum., Method (C) is novel constant center spectrophotometric method (CC) Method (D) is mean centering of the ratio spectra (MCR) at 284 nm. The calibration curve is linear over the concentration range of 10–80 and 4–40 μg/ml for AM and AT, respectively. These methods are tested by analyzing synthetic mixtures of the cited drugs and they are applied to their commercial pharmaceutical preparation. The validity of results was assessed by applying standard addition technique. The results obtained were found to agree statistically with those obtained by a reported method, showing no significant difference with respect to accuracy and precision.
Biography:
Miranda Younes is an Assistant Professor at P. University in Alexandria, Egypt.
Abstract:
Two simple, precise and stability-indicating High-Performance Liquid Chromatography (HPLC) and High-Performance Thin-Layer Chromatographic (HPTLC) methods were introduced and compared for the simultaneous determination of Coenzyme Q10 (Q) and Vitamin E (E) ; alpha-tocopherol acetate, in tablet formulation. The HPLC separation was achieved on a C18 column using Acetonitrile: Tetrahydrofuran: Water (55:40:5, v/v/v) as mobile phase at a flow rate of 0.7 mL/min at ambient temperature. The quantitation was achieved UV detection at 280 nm over the concentration range 5 – 60 and 50 - 200 μg/mL for Q and E, respectively. For HPTLC method, Q and E were chromatographed on silica Gel 60 F254 TLC plate using Benzene: Chloroform (7: 3, % v/v) as mobile phase and then scanned at 280 nm. Linearity is in the range of 0.3 - 2 and 0.5 – 5 μg/band for Q and E, respectively. The two methods were validated according to ICH guidelines and different chromatographic parameters were optimized for adequate determination of Q and E in the tablet. Furthermore, a forced degradation study of Q and E was done under various conditions including; hydrolysis (acid, alkaline and neutral), oxidation, thermal and photo-decomposition. Statistical analysis proved that the two methods were precise, accurate, selective and economical with no significant difference between them. They may be used for routine simultaneous estimation of Q and E in tablets.
Md. Zakir Sultan
University of Dhaka, Bangladesh
Title: A novel ion-pair RP-HPLC method for simultaneous quantification of naproxen and esomeprazole in pharmaceutical formulations
Biography:
Md. Zakir Sultan has completed his PhD from Kongju National University, South Korea and postdoctoral studies from University of Dhaka, Bangladesh. He is a Senior Scientist, Centre for Advanced Research in Sciences (CARS), University of Dhaka, Bangladesh. He has published 4 books and more than 48 papers in reputed journals and has been serving as an editorial board member of the Journal of Scientific Research.
Abstract:
A rapid, sensitive and stability indicating ion-pair reversed-phase high-performance liquid chromatographic method was developed for simultaneous estimation of naproxen (NPX) and esomeprazole (ESP) in pharmaceutical preparations. In our study, this new method was used to overcome the instability problem of ESP during high performance liquid chromatographic analysis in the presence of acidic drugs such as NPX. The method was validated according to ICH, FDA and USP guidelines with respect to accuracy, precision, specificity, linearity, solution stability, robustness, sensitivity and system suitability. The method was developed by using an isocratic condition of mobile phase comprising buffer [tetrabutylammonium hydroxide (0.0077 M) and n-heptane sulfonic acid–Na salt (0.002 M), pH 7.6], acetonitrile and methanol in a 60:20: 20 v/v/v ratio at a flow rate of 1.5 mL/min over a C-18 (Octadecyl-silica, 5 mm, 250 3 4.6 mm) column at ambient temperature. The recovery for both drugs was found to be >99% which demonstrated the accuracy of this method. Intra- and inter-day precision studies of the new method were less than the maximum allowable limit [% relative standard deviation (RSD) ≤ 2.0 according to FDA]. The method showed linear response with a correlation coefficient (r2) value of 0.999 for both drugs. More importantly, ESP was quite stable in diluting solvent and mobile phase in the presence of NPX for >3 days. Therefore, it was found to be an accurate, reproducible, sensitive and highly stability-indicating method and can be successfully applied for routine analysis of simultaneous assay of NPX and ESP in pharmaceutical dosage forms.
Mahnaz Farahani
National Institute of Standards and Technology, USA
Title: Unexpected peaks in tandem mass spectra due to reaction of product ions with residual water in mass spectrometer collision cells
Biography:
Mahnaz Farahani has completed his PhD from American University and Postdoctoral studies from National Institute of Standards and Technology. She is the senior reviewer at FDA. She has published more than 25 papers in reputed journals.
Abstract:
Rationale: Certain product ions in electrospray ionization tandem mass spectrometry are found to react with residual water in the collision cell. This reaction often leads to the formation of ions that cannot be formed directly from the precursor ions, and this complicates the mass spectra and may distort MRM (multiple reaction monitoring) results. Methods: Various drugs, pesticides, metabolites, and other compounds were dissolved in acetonitrile/water/formic acid and studied by electrospray ionization mass spectrometry to record their MS2 and MSn spectra in several mass spectrometers (QqQ, QTOF, IT, and Orbitrap HCD). Certain product ions were found to react with residual water in collision cells. The reaction was confirmed by MSn studies and the rate of reaction was determined in the IT instrument using zero collision energy and variable activation times. Results: Examples of product ions reacting with water include phenyl and certain substituted phenyl cations, benzoyl-type cations formed from protonated folic acid and similar compounds by loss of the glutamate moiety, product ions formed from protonated cyclic siloxanes by loss of methane, product ions formed from organic phosphates, and certain negative ions. The reactions of product ions with residual water varied greatly in their rate constant and in the extent of reaction (due to isomerization). Conclusions: Various types of product ions react with residual water in mass spectrometer collision cells. As a result, tandem mass spectra may contain unexplained peaks and MRM results may be distorted by the occurrence of such reactions. These often unavoidable reactions must be taken into account when annotating peaks in tandem mass spectra and when interpreting MRM results.
Doo Soo Chung
Seoul National University, Korea
Title: Speciation of trace arsenic compounds in drinking water with counter-flow electrokinetic supercharging
Biography:
Doo Soo Chung has completed his PhD from Harvard University and Postdoctoral studies from MIT and Iowa State University. He has published more than 100 papers in reputed journals. He has been working on a variety of topics ranging from the realization of molecule optics which controls the motion of molecules with light and Bioanalytical techniques such as Capillary Electrophoresis
Abstract:
Chronic ingestion of arsenic in water may cause various diseases, including cancer and keratosis. A guideline for arsenic in drinking water has been set at 10 ppb of total arsenic by the World Health Organization (WHO). However, inorganic forms of arsenic, such as arsenites [As(III)] and arsenates [As(V)], are much more toxic than the organic forms as monomethylarsonic acid (MMA) and dimethylarsinic acid (DMA). Hence the quantitation of specific arsenic species may be more meaningful than the total arsenic determination for the evaluation of the health risks from arsenic-contaminated drinking water. We report a highly sensitive way of arsenic speciation using a commercial Capillary Electrophoresis (CE) instrument equipped with a UV absorbance detector. We used a counter-flow electrokinetic supercharging technique to enhance the detection sensitivity. Electrokinetic supercharging is one of the most powerful sample stacking methods that combines field amplified sample injection and transient isotachophoresis. In counter flow electrokinetic supercharging, a constant counter pressure is applied during sample injection in order to counterbalance the movement of the injected sample zone, obtaining a pronounced increase in the amount of sample injected and the portion of the capillary available for electrophoresis.
Mohamed M Hefnawy
King Saud University, Saudi Arabia
Title: Estimation of enantioresolution of multiple stereogenic drugs using mobilized and/or immobilized polysaccharide-based HPLC chiral stationary phases
Biography:
Prof. Dr. Hefnawy got his PhD and postdoctoral studies from School of Pharmacy, University of Georgia, Athens, GA, U.S.A. He was supervisor of nine PhD and 15 MSc dissertations. He has published more than 97 papers in international journals and has been serving as an editorial board member of repute.
Abstract:
Enantioseparation of drugs with multiple stereogenic centers is challenging. This study objectives to evaluate the efficiency of different mobilized and/or immobilized polysaccharide-based chiral stationary phases to separate enantiomers of some drugs containing multiple stereogenic centers namely indenolol, nadolol, labetalol. The critical mobile phase variables (composition of organic solvents, acid/base ratios) were carefully studied to compare the retention time and elution order of all isomers. Different chromatographic parameters such as capacity factor (k), selectivity (α) and resolution (Rs) were calculated. In this presentation the experimental conditions and the possible chiral recognition mechanisms will be discussed.
Kamlesh Shirvas
Guru Ghasidas University, India
Title: MALDI-MS imaging of low molecular weight biomolecules in mouse brain using TiO2 nanoparticles
Biography:
Dr. Kamlesh K. Shrivas obtained his Master degree in Chemistry (2000) and Ph.D. degree in Chemistry (2004) from Pt. Ravishankar Shukla University, India. He worked as a quality control officer, Gharda Chemical Ltd., India (2005-2006). He worked as a post doctoral fellow at Tamkang University, TAIWAN and National Sun-Yat Sen University, TAIWAN (2006-2008), at Food Drug and Administration, USA (2008-2009), at Hamamatsu University School of Medicine, JAPAN (2009-2011). Since 2009, he has been an Assistant Professor at the Guru Ghasidas University, India. His current interests include the analytical development of chemical sensors for the detections of metal ions, drugs and biomolecules. In addition, imaging and identification of biomolecules (peptides, proteins, lipids and drugs) in tissue sample using mass spectrometry. He has published 44 research papers in highly impact journals and 3 book chapters.
Abstract:
The presence of low molecular weight biomolecules in brain has important role in biosynthesis, product degradation, energy production, signaling and defense. Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) has proven to be one of the most powerful tools available to researchers in biochemistry and molecular biology for the identification of biomolecules in various types of biological samples. We report the detection of a group of endogenous low molecular weight metabolites in mouse brain (80-500 Da) using TiO2 nanoparticles (NPs) without any washing and separation step prior to MS analysis. This approach is a simple, inexpensive, washing and separation free for imaging and identification of low molecular weight biomolecules in mouse brain. The white and gray matter of the mouse brain is differentiated by the ion distribution pattern of biomolecules found in the tissue section. We believe that the biochemical information from distinct regions of the brain using a MALDI-MS imaging will be helpful in elucidating the imbalances linked with diseases in biomedical samples.
Yuegang Zuo
University of Michigan-Dearborn, USA
Title: Determination of Creatinine, Uric and Ascorbic Acid in bovine milk and orange juice by hydrophilic interaction HPLC
Biography:
Yuegang Zuo is a Full Professor in analytical and environmental chemistry and Director of Graduate Programs at Department of Chemistry and Biochemistry, University of Massachusetts Dartmouth. He received his Ph.D. from Swiss Federal Institute of Technology Zurich in 1992. Most of his recent research has focused on separation, identification and quantification of endocrine disrupting pollutants, phenolic antioxidants and their metabolites in plants, human foods, liquids, and in the related environments and examine their occurrence, sources, distribution, transportation, bioeffects and fate in the biochemsphere. He has published over 70 peer-reviewed papers in prestigious international scientific journals such as Science, and ES&T.
Abstract:
Creatinine (Cr), uric (UA) and ascorbic acid (AA) are common constituents in human fluids. Their abnormal concentrations in human fluids are associated with various diseases. Thus, apart from the endogenous formation in human body, it is also important to examine their sources from food products. These polar organic molecules have very limited retention on commonly used reversed-phase HPLC columns [Zhou et al., Analytical Methods 5 (2013) 1307-1311; Y. Zuo, 2014, High-Performance Liquid Chromatography (HPLC): Principles, Procedures and Practices. Nova Science Publishers, Inc., New York]. In this study, a rapid and accurate HILIC method was developed for simultaneous determination of Cr, UA and AA in bovine milk and orange juice. Milk samples were pretreated by protein precipitation, centrifugation and filtration, followed by HPLC separation and quantification using a Waters Spherisorb S5NH2 column. The developed method has been successfully applied to determine the concentration of UA, AA and Cr in milk and fruit juice samples. The milk samples tested were found to contain UA and creatinine in the concentration range of 24.1-86.0 and 5.07-11.2 µg mL-1, respectively. The orange juices contain AA over 212 µg mL-1.
Sigrid Mennickent
University of Concepción, Chile
Title: Quantitative determination of Topiramate in human serum and umbilical cord blood
Biography:
Mennickent S is a Pharmacist, Master of Pharmaceutical Sciences, Associate Professor at University of Concepción, Chile. She has published more than 48 papers in reputed journals (ISI), 7 book chapters, and has been serving as reviewer and as Editorial Board Member reviewer in many prestigious journals. She has been invited as speaker in some Scientific Congress in different countries.
Abstract:
A simple, rapid, specific, precise and accurate LC/DAD method for quantification of topiramate in human serum and cordon umbilical cord blood was developed and validated. The method includes a liquid-liquid extraction of the analyte from the both matrix using a dichloromethane as extraction solvent. Dabsil was used as rderivatization compound. In the developed method, chromatography was performed using acetonitrile: acetate buffer (60:40, v/v) as mobile phase, with a flow of 1 mL/min, and Purosphere ® C10 column (5 uµ). Detection was done at 254 nm. The regression data for the calibration plots showed good linear relationship (r=0.999) for both matrix, in the range of 2.0 and 40.0 ug/mL, in human serum (therapeutic range concentration: 5.0-20.0 ug/mL), and between 1.6 and 50.0 ug/mL for umbilical cord blood. The % RSD of intra-assay and inter-assay precision, were in the range of 0.98% to 2.97% (n=3) and 1.06% to 3.54% (n=9), respectively, for human serum, and 1.21% to 3.58% (n=3) and 2.10% and 3.68% (n=9), for umbilical cord blood. Recovery values were between 94.72% and 98.96% (RSD ≤4.25), for both matrix, respectively. Patient serum samples and umbilical cord blood samples were analyzed by this method successfully. Therefore, this LC/DAD method is suitable for quantitative determination of topiramate in these biological fluids.
Vladimir Shulaev
University of North Texas, USA
Title: Application of novel analytical techniques and data analysis tools for comprehensive metabolomics analysis of complex biological mixtures.
Biography:
Professor Vladimir Shulaev holds dual Ph.Ds. in Biological Sciences and Plant Biology and has over 30 years research experience working at academia and industry. At UNT he established the state of the art Metabolomics facility and heads the Center for Metabolomics and Metabolic Signaling Research and Biochemical Profiling Group. His research group develop novel analytical techniques for both targeted and non-targeted metabolomics using mass spectrometry platforms and applied metabolomics platform to systems biology, gene function elucidation, cancer development andprogression,andmodelingandsimulationofbiologicalnetworks.
Abstract:
Metabolomics analysis of complex mixtures using a single separation technique is challenging due to the diversity of metabolites polarity, volatility and the large range of concentrations in biological samples. We examined two novel analytical approaches for comprehensive analysis of metabolites in various biological matrices. First approach is based on Ultra Performance Convergence Chromatography (UPC2), a chromatographic system that utilizes liquid CO2 as primary solvent to leverage the chromatographic principles and selectivity of normal phase chromatography while providing the ease-of-use of reversed-phase LC. We utilized the sub-2µm particle supercritical chromatography for the separation of free fatty acids, neutral and polar lipids in a single lipid extract. Second approach utilizes Atmospheric pressure GC (APGC) is a ‘soft‘ chemical ionization technique that generates a spectrum conserving the molecular ion species with minimal fragmentation, which differentiates it from traditional vacuum source GC-MS based on electron ionization. We combined APGC with ion mobility (IM) to enhance peak capacity and improve selectivity and specificity of analysis. We demonstrated the utility of APGC-TOF-MS for metabolomics analysis of various mutant plant genotypes. Raw data were analyzed using TransOmics software that adopts an intuitive workflow approach to performing comparative metabolomics and lipidomics data analysis. The workflow starts with raw data file loading, then retention time alignment and deconvolution, followed by analysis that creates a list of features. The features are then identified with compound searches and explored using multivariate statistical methods.
Yuhui (Henry) Zhao
Epcor Water Canada, Canada
Title: An auto self-cleaning filter for on-line analyzers
Biography:
Yuhui (Henry) Zhao completed his PhD in Analytical Chemistry from the University of Alberta in 1995. He has been working in a few analytical laboratories for the past 20 years as a Senior Scientist. His research and development interests cover the areas of Inductively Coupled Plasma (ICP)-Optical Emission Spectroscopy, ICP-Mass Spectrometry, GC and GC-Mass Spectrometry. He is currently working as a QA Scientist at Epcor Water Service Inc., Edmonton, Alberta, Canada.
Abstract:
In most water treatment plants, there are many on-line analyzers. Line clogging, especially in the run-off time each year, has always been a very serious problem for these analyzers. It often makes the measurement very difficult, if not impossible. In order to eliminate the clogging problem, an auto-self-cleaning filter has been designed, constructed, and tested. The design and construction was done in house. To keep the cost as low as possible, most of the material and parts used in this project were harvested from old equipment. The filter consists of a Y-shaped strainer with one two-way and one three-way solenoid valve. It is fully automatic and functioning as the following: Raw water get into the Y-shaped strainer, clean water passing through the screen and directed into the monitoring devices (the Analyzer) at programmed times; Sediment accumulated in the other branch of the Y- shaped strainer is drained at programmed moments; The filter is then self-back-washed by a clean water stream after receiving a signal from the analyzer. Each function-change is indicated by a color-changing LED and the function of each operation stage is displayed on an LCD panel. This filter can be used for sample-conditioning on on-line GC for raw water analysis. Modified / simplified version of this device can be used for any other on-line analyzers. It will prevent the line clogging, reduce instrument down-time, enhance data quality and further contribute to drinking water safety.
Biography:
Makhapa Makhafola is currently the General Manager: Research & Development at Mintek. He worked as Lecturer in Analytical Chemistry at Technikon Northern Gauteng (now called Tshwane University of Technology) and University of Venda. In 2004 he was appointed Director: Quality Assurance at Border Technikon (now called Walter Sisulu University). He was the Director: Quality Assurance at the University of Venda until he joined University of Kwa-Zulu Natal as the Director Quality Promotion & Assurance in July 2010, part of his responsibility was to lead the World University Rankings project. He served as member of Umalusi Council and also as Chairperson of Lovedale FET College Audit Committee. He is currently the Chairperson of DST/MINTEK Nanotechnology Innovation Centre Steering Committee. He served as a member of the Higher Education Quality Assurance Manager’s Forum and also chaired and facilitated various workshops on quality assurance in higher education. He is also serving as an academic committee member of QS World Ranking Universities. He did the post-doctoral training in Analytical Chemistry at Indiana University. He presented his research work in more than 19 international conferences and published in credible journals.
Abstract:
The mandate of Mintek is set out in the Mineral Technology Act, which is to serve the national interest through Research, Development and Technology Transfer, to promote mineral technology, and to foster the establishment and expansion of industries in the field of minerals and products derived there from. Mintek is a Research and Development (R&D) organisation that specialises in applied research in various scientific and engineering fields and we are publicly (50%) and privately (50%) funded. Mintek provides world class research and development expertise, testwork, and process optimization for the mining industry locally and internationally. The activities range from initial bench-top investigations to full process flow sheet development and the design, construction, commissioning, and optimization of industrial plants The ultimate measure of Mintek’s success is the extent to which R&D activities contribute towards enhancing productivity, economic growth and socio-economic development of South Africa using minerals. This paper present some of Mintek’s biggest achievements which include the commissioning of the rear earth pilot plant, Cynoprobe cyanide analyser, completion of the nanotechnology clean room facilities, dry sorting technology, rehabilitation of derelict and ownerless mines, etc.
Biography:
Jana Jaklová Dytrtová completed her studies at several well-established Universities in the fields of: Physical Chemistry, Environmental Chemistry, Agricultural Chemistry, Forest Engineering, and Biology and Arts. She finished her PhD in 2008 and after her maternity leave she was a Post doc of Dr. Detlef Schröder and became specialist in mass spectrometry. She has published more than 30 papers in reputed journals and has been serving as a reviewer for 5 scientific journals. She is a member of 3 scientific societies. She works as a scientist in a public research institution.
Abstract:
Detection of pesticides in environmental matrices is neither for the modern analytical chemistry routine operation. It can be used, among others, to reveal their behavior in the environment. Electrochemical separation of chemicals is a powerful innovative tool that can extend the range of hyphenation methods in combination with mass spectrometry. Applicability of newly developed electrochemical separation using silver ions is based on high reactivity of nascent silver ions and on the high affinity of Ag+ to small ligands containing N. This group also includes the triazole fungicides, e.g. cyproconazole (Cyp). In this case, the generated Ag+ subsequently forms complex [Ag(Cyp)]+, detectable by electrospray ionization mass spectrometry. The newly developed method of separation prior ESI MS generally relates to (i) the detection of metal complexes with pesticides, (ii) the trace analysis of pesticides in environmental matrices, and (iii) the determination of the complexes stability and stoichiometry.
Jacek Namieśnik
Gdansk University of Technology, Poland
Title: Application of passive sampling techniques as a usable tool in the field of environmental quality monitoring
Biography:
Jacek Namiesnik is the Dean of Chemical Faculty and the Professor of Analytical Chemistry at the Gdansk University of Technology, Poland. He is the Chairman of the committee of analytical chemistry of the Polish Academy of Sciences (PAS) and also the Member of the State Commission for evaluation of scientific degrees and titles. His research interests include new techniques of extraction of analytes from different types of samples and new procedures for determination of a wide range of analytes from samples characterized by the complex composition of the matrix.
Abstract:
Analysis of literature data published for the past 20 years leads to the conclusion, that passive sampling technique has been developing very quickly and is commonly used in the field of monitoring pollutants in air, water and soil environment. The popularity of application of passive sampling techniques in analytical and environmental chemistry results from its many advantages e.g.: Simplicity in use, low costs of exploitation, no need for expensive and complicated equipment, no power requirements, the ability to produce accurate results. Over the last decade in Department of Analytical Chemistry, Gdansk University of Technology passive sampling technique has been applied in different areas of environmental analysis and monitoring, such as: • Screening studies and source identification - determination of occurrence or identification (qualitative or semi quantitative) of pollutants present in both atmospheric and indoor air; • Determination of pollutant concentration (BTEX concentrations) in the environment (quantitative) - integration of ambient concentrations of pollutants over time scales: short-time scale (hours/days) and long-time scale (weeks/months/years). The shorter time-scales facilitate studies of pollutant dispersal, fluxes and transport processes. Long time-scales would allow the identification of source/sink regions and underlying trends in ambient levels; • Mapping the ambient distribution of BTEX (mapping concentrations) to yield input data for regional distribution models - visualization of the spatial distribution of pollution levels in the form of maps allows for sophisticated mathematical analysis and proper decision-making concerning the environment; • Human exposure assessment - personal monitoring with passive sampling - the most accurate estimate of a personal ‘true’ exposure; • Screening (non-invasive) in-situ studies on the emission flux of organic compounds (mainly VOCs) emitted from indoor materials, using miniaturized passive emission chambers. This kind of devices combines the features of classical emission chambers in a miniaturized form (miniaturized passive emission chambers), with the advantages of a passive sampling technique (low cost of particular construction elements, possibility of handling by unqualified staff).
- Track:9 Applications of Analytical and Bio analytical Methods
Track:10 Diagnostic Assays and Test Kits
Track:11 New Instrumentation and Equipment
Session Introduction
Kabir Hussain
MedImmune, UK
Title: Immunoassays in drug development – Comparing and contrasting different platforms
Biography:
Kabir Hussain has a BSc degree in Cancer Biology & Immunology from the University of Bristol, UK. As part of the Clinical Pharmacology and DMPK group, he is responsible for the development, validation and execution of Immunoassays used in the analysis of samples from clinical and non-clinical studies, including GLP toxicology studies. He works in a GLP certified laboratory and consequently has experience working in accordance with the OECD principles of Good Laboratory Practice. He is a Principle Investigator for GLP multi-site studies and a Study Director for method validation studies. He previously worked at a CRO developing and validating immunoassays.
Abstract:
The drug development process requires sensitive and robust assays to enable PK/PD and Immunogenicity assessment of biologics. Immunoassays are commonly employed to this purpose, utilising the target-specific properties of antibodies to detect the analyte of interest. With the rapid advances in technology, the range of immunoassay platforms available is growing whilst existing platforms are becoming more refined, enabling bioanalytical scientists to choose a platform that is tailored to their needs. Various factors can influence the choice of platform, these can be either assay related such as sensitivity or assay type (quantitative/immunogenicity), or more general factors such as time and cost effectiveness. The Meso Scale Discovery® platform has become established as a reliable platform, particularly for immunogenicity assessment whereby a homogenous solution phase incubation of the sample with the capture and detection reagents provides a simple, easy to perform assay. The GyrolabTM is a largely automated platform that provides a time effective option allowing quick turnaround for analysis of samples. The CiraTM immunoassay platform from Aushon Biosystems, Inc. provides highly sensitive assays that are ideal for Biomarker analysis and also specialises in multiplexing capabilities, making it an attractive option for pharmacodynamics assessment. Carefully selecting the appropriate platform can improve the likelihood of successfully navigating biologics through the drug development process.
Lihan Tan
Bioprocessing Technology Institute, Singapore
Title: Measurement of histones in mammalian cell culture harvest
Biography:
Lihan Tan has completed her PhD from National University of Singapore, Singapore. Currently, she is working as a research scientist in downstream processing group, Bioprocessing Technology Institute, A*STAR, Singapore. Her research interest is on developing new platforms for protein or virus purifications, and on analytical techniques to characterize the biomolecular interactions/contents in mammalian cell culture harvest.
Abstract:
Histones, in the form of chromatin heteroaggregates in cell culture harvests, are becoming recognized as a major challenge in antibody purification. However, recent publications have shown that immunoassays for host cell proteins do not detect histones. In addition, histone standards provided in commercially available histone ELISA kits are not based on authentic CHO histones. This presentation will show how to prepare authentic host cell histone standards, and how they are necessary for accurate quantitation of histone contamination in cell culture harvests and during purification procedures.
Biography:
Faiz Ali Post Doctoral Fellow advanced separation sciences, graduate school department of chemistry and chemical engineering, Inha University, South Korea.
Abstract:
Miniaturized partially sub-1µm porous silica monolith particles with large pore size, exhibiting excellent chromatographic performance have been synthesized in a relatively large scale by an elaborated sol-gel procedure. The resultant silica monolith particles were chemically modified with chloro-dimethyl-octadecylsilane (C18), and end-capped with a mixture of hexamethyldisilazane and chlorotrimethylsilane. Very good separation efficiency (185,000/m) and resolution (6.05) were achieved when the C18-bound phase was evaluated for a test mixture of five benzene derivatives after packing in a stainless steel column (1.8 mm × 150 mm). The optimized elution conditions were found to be 70/30 (v/v) acetonitrile/water with 0.1% TFA at a flow rate of 25µL/min. The column was also evaluated for fast HPLC analysis using a flow rate of 100µL/min where all the five analytes were eluted in three minutes with still reasonable efficiency (Ca. 96000/m) and excellent resolution within the pressure limit of conventional HPLC. The strategy of particle size reduction combined with C18 modification along with large pores and monolithic architecture has resulted in a useful stationary phase (C18-bound silica monolith particles) of low production cost showing excellent chromatographic performance capable of fast HPLC analysis.
Alberto Chisvert
University of Valencia, Spain
Title: Cosmetic ingredients: From the cosmetic to the human body and the environment
Biography:
Alberto Chisvert (BSc, Faculty of Chemistry, University of Valencia, 1999; PhD, Dept. of Analytical Chemistry, University of Valencia, 2003). He is Associate Professor of the Dept. of Analytical Chemistry of University of Valencia. At present, he has published more than 60 articles in reputed journals, and more than 10 book chapters. Moreover, he is the Co-Editor of the book Analysis of Cosmetic Products (Elsevier, 2007). He is Editorial Board Member of Advances in Analytical Chemistry, American Journal of Analytical Chemistry, Chromatography Research International, International Journal of Analytical Chemistry and Journal of Trace Analysis in Food and Drugs, and is a regular reviewer of more than 27 journals. His research areas are focused on both liquid and gas chromatography coupled to mass-spectrometry, liquid- and solid-phase microextraction, in bioanalysis and cosmetic, pharmaceutical and environmental analysis.
Abstract:
Current social demand for cosmetic products in the developed countries has prompted the cosmetics industry to achieve a leading position within the chemical industry. That is why health authorities are increasingly concerned by this sector, regulating these products, not only to ensure the safety of users but also to ensure their effectiveness. Therefore, quality control in the cosmetic industry is demanded. However, there are not reference analytical methods enough to cover the needs of this sector, so the cosmetics industry is continually requiring the development of analytical methods. Moreover, prohibited substances could be present at trace levels, as they are not intentionally added to the product, but are formed during the manufacturing process. Therefore sensitive analytical methods, in addition to accurate and precise, are needed. Furthermore, studies show that some cosmetic ingredients can be absorbed through the skin and penetrate into the human body, which can be metabolized and/or excreted and even bioaccumulated. Studies are rather scarce in this field as a result of having not analytical methods for determining the cosmetic ingredient and its metabolites at trace levels in biological fluids. Finally, there are several studies that show that cosmetic ingredients reach the aquatic ecosystem causing adverse effects on flora and fauna. Therefore the development of sensitive analytical methods for determining cosmetic ingredients at trace levels on the environment is of great interest from the surveillance environmental point of view. The purpose of this presentation is to provide an overview of the analytical contributions of our group in this three interesting fields.
Katarzyna Owczarek
Gdańsk University of Technology, Poland
Title: Assessment of cytotoxic and endocrine potential of selected xenobiotics commonly present in food products
Biography:
Katarzyna Owczarek has completed her MSc studies in 2014 from Chemical Faculty Gdansk University of Technology. She started PhD studies on Department of Analytical Chemistry in Gdansk University of Technology in 2014. She has published 3 papers in reputed journals and 1 monograph.
Abstract:
More and more increased intensity of anthropo-pressure processes can be observed, among other things, in the release of great quantities of synthetic substances into the environment, including the Endocrine Disrupting Compounds (EDC). A vast number of chemical substances are considered to belong to the this group, including substances which occur naturally in the environment, such as mycotoxins and phytoestrogens, and substances which have been artificially released to the environment as a result of human activities (synthetic hormones, growth promoters, phthalates, bisphenols, metals, pharmaceutical residues, organo-chlorine compounds including PCBs). Taking into account the plurality and chemical diversity of the aforementioned compounds, it should come as no surprise that these substances are almost ubiquitous in various elements of the environment and in the food chain, in which they are subjected to bio-magnification. These chemicals are mainly suspected to contribute to the induction of neoplastic diseases such as the breast and prostate cancer, metabolic diseases, including obesity, genetic modifications and impairment of reproductive functions. They are also suspected to have mutagenic and cytotoxic effects and that they disrupt regulatory pathways of some organs. That is the reason why all attempts to broaden the knowledge of relations and mechanisms triggering the development of relevant diseases are justified. The aim of the conducted research was to evaluate cytotoxicity and endocrine potential of selected EDCs which cause serious food contamination (bisphenol A, 4-nonynphenol, 4-t-octylphenol, diethylstilbestrol, bisphenol A diglycidyl ether and its derivatives and phthalates) with MTT and YES/YAS assays.
Biography:
José Manuel Herrero-Martínez obtained his PhD in Chemistry at the University of Valencia, Spain, in 1996. He worked in teaching and research at the Universities of Amsterdam and Barcelona. Since 2009, he is working as an Associate Professor in the Department of Analytical Chemistry at the University of Valencia. His research interests focus on the development of polymeric materials, in particular polymer monoliths, with application in separation science.
Abstract:
Along the last years, monolithic porous polymers have been widely investigated as stationary phases for separation techniques. Advantages are ease of preparation, high permeability and a vast variety of easily modifiable surface chemistries. Nevertheless, these monoliths have low surface areas due to the lack of an adequate mesoporous structure. Adverse consequences are low retention and reduced sample load capacity. The development of hybrid materials containing nanoparticles (NPs) has opened promising ways of overcoming these limitations, also enormously expanding the available monolith classes. We have work with two approaches, namely to modify the surface of methacrylate monoliths with covalently bonded NPs (I), and to obtain monoliths by copolymerization with derivatized NPs (II). Concerning approach I, the surface of monoliths was first modified with thiol-containing ligands, thus to be able of bonding silver NPs (AgNPs) on them. Monoliths with very large reactive surfaces resulted. The bonded AgNPs showed a large capacity of strongly retain thiol-containing analytes. Further, the retained analytes were easily eluted by replacement with compounds also containing thiol groups if present in excess in the mobile phase. Application to the pre-concentration of thiol-containing compounds (glutathione, N-acetylcysteine, peptides and proteins) in biological fluids and food was demonstrated. Regarding to approach II, iron oxide NPs were first silanized with a vinyl-containing reagent. The resulting vinylized NPs were copolymerized with methacrylate monomers to render a monolith with a rough surface. This monolith exhibited enhanced retention of a variety of molecules (e.g., organophosphorous pesticides), as well as an improved efficiency.
Shobini Jayaraman
Boston University School of Medicine, USA
Title: Comprehensive overview of biophysical studies of lipoprotein stability
Biography:
Shobini Jayaraman has completed her PhD from Indian Institute of Technology, Madras, India and Postdoctoral studies from Weizmann Institute of Science, Israel. She was the recipient of Sir Charles Clore fellowship at Weizmann Institute of Science. Currently she is a Senior Research Scientist at Boston University School of Medicine. She serves as the liaison for academic and industrial contract research services at Boston University. She has published more than 25 papers in reputed journals. Her recent publication in JBC has been chosen as paper of the month in May-2014 by International Atherosclerosis Society.
Abstract:
Lipoproteins are nanoparticles comprised of proteins and lipids that provide vehicles for transport of fat and cholesterol in circulation. High levels of certain lipoproteins increase the risk of heart disease. Each lipoprotein is a non-covalent assembly of several proteins and several hundred lipids. The major challenge in the biophysical analysis of lipoproteins arises from their heterogeneity in size (7-100 nm), density (1.06-1.22 g/L), and protein and lipid composition. Moreover, lipoproteins are highly dynamic assemblies undergoing continuous remodeling via various enzymatic and non-enzymatic reactions. This provides a major challenge for detailed structural studies of lipoproteins. To overcome this challenge, we designed an integrated biophysical approach by combining far- and near-UV circular dichroism (CD) spectroscopy, turbidity, differential scanning calorimetry (DSC), fluorescence spectroscopy, transmission electron microscopy (EM), size-exclusion chromatography (SEC) and other methods to analyze the structure and remodeling of all major lipoprotein classes. This integrated approach was used to study thermal denaturation of human low- and high-density lipoproteins (LDL and HDL, or bad and good cholesterol). The results clearly showed that lipoprotein stability is controlled by kinetics barriers. Interestingly, heat-induced remodeling of all lipoproteins involves partial protein unfolding/dissociation and lipoprotein fusion and rupture. These structural transitions mimic key aspects of in-vivo lipoprotein remodeling. These and other emerging approaches will allow one to study structural, dynamic and functional properties of larger more challenging systems. Ultimately, such integrated approaches are hoped to bridge the gap between the biophysical studies of isolated macromolecules or their complexes, and the complexity of cellular systems.
Yeun-Jun Chung
The Catholic University of Korea, Korea
Title: Stuffer-free multiplex ligation-dependent probe amplification technology for identifying the copy number variation markers associated with the risk of SLE
Biography:
Yeun-Jun Chung is a Professor of Medical Microbiology and Head of Integrated Research Center for Genome Polymorphism (Catholic Medical College, Korea). He has studied genomics based personalized medicine for past twenty years. Since he founded IRCGP, he has studied Variomics and Oncogenomics using array-CGH, GWAS, NGS, MLPA-CE-SSCP and BI tools. He has published >80 research papers including Gastroenterology, Genome Res, Arthritis Rheum, Cancer Res, Bioinformatics, J Pathol etc. Currently he is an editor-in-chief of Genomics & Informatics (official journal of Korean Genome Organization) and an editorial board member of Experimental & Molecular Medicine and World Journal of Gastroenterology.
Abstract:
Copy number variation (CNV) is one of the major components of human genetic variations and it is thought to contribute to inter-individual differences in diverse phenotypes. Several CNVs have been suggested to be associated with systemic lupus erythematosus (SLE) through the target gene approach; however, genome-wide feature of CNVs in the risk of SLE has not well studied. Recently, our group studied the genome-wide CNVs with 964 SLE patients and 711 normal control individuals by whole genome SNP array analysis, and identified three CNVs of RABGAP1L, 10q21.3, and C4 associated with the risk of SLE in Korean Women (Arthritis and Rheumatism 65:1055-63 2013). For the multiplex detection of the 3 CNVs, we adopted a stuffer-free multiplex ligation-dependent probe amplification based on conformation-sensitive capillary electrophoresis, which we developed previously (MLPA-CE-SSCP, Electrophoresis 33, 3052–61 2012). For the MLPA-CE-SSCP based SLE CNV detection system, we also included 3 previously reported CNVs such as CCL3L1, FCGR3B, and EGR2. As a result, all the targets were well separated and the expected CNVs were consistently identified by the SLE MLPA-CE-SSCP system. After validation of the performance of SLE MLPA-CE-SSCP system, we applied the system for 113 SLEs and found that deletion of C4 was the most significantly associated with the risk of SLE. Our results suggest that the SLE MLPA-CE-SSCP system can be useful for predicting the risk of SLE. However, further larger scale study will be required.
Albert Van Den Berg
BIOS/Lab on a Chip group, The Netherlands
Title: Labs, cells and organs on a chip
Biography:
Albert Van Den Berg received his MSc in applied physics in 1983, and his PhD in 1988 both at the University of Twente, the Netherlands. From 1988-1993, he worked in Neuchatel, Switzerland, at the CSEM and the University (IMT) on miniaturized chemical sensors. In 2000 he was appointed as full professor on Miniaturized Systems for (Bio) Chemical Analysis in the faculty of Electrical Engineering. He received several honors and awards such as Simon Stevin (2002), ERC Advanced (2008) and ERC Proof of Concept (2011, 2013) grants, Spinoza prize (2009), Honorary University Professorship (Twente, 2010), and board member of the Royal Dutch Academy of Sciences (KNAW) in 2011.
Abstract:
The recent rapid developments in microfluidic technologies have enabled the realization of miniaturized laboratories. This labs-on-a-chip will play an important role in future medicine, both in point-of-care devices for drug or biomarker monitoring, as well as in early diagnostic devices. We developed a pre-filled ready-to-use capillary electrophoresis platform for measuring ions in blood. It is used to monitor lithium in finger-prick blood of manic-depressive patients, but can also be used for measuring calcium in blood for prevention of milk fever, or for measuring creatinine in blood or sodium in urine for early detection of ESRD. Apart from diagnostic devices, microfluidic devices are increasingly used to realise advance disease and organ-models, as illustrated by the blood-brain barrier chip and a blood vessel on a chip. Finally, a microdroplet platform for encapsulation of single cells in microdroplets, ordering of these microdroplets and 1:1 fusion of these droplets is demonstrated. We believe this is a very powerful new tool that can be used for high-throughput single cell experimentation.
Biography:
Cyril Pernot is working in the compound-semiconductors field since 1997. He received a PhD degree in Physics from the University of Montpellier in 2000 for his work on AlGaN-based UV-photo-detectors. Since 2007 he is involved in the development of AlGaN-based UV-LED and he is currently in charge of the crystal growth section at NIKKISO Co., Ltd for the production of UV LED epitaxial wafers.
Abstract:
Deep UV LEDs (255-350 nm AlGaN-based UV-LED) have many advantages compare to conventional UV lighting such as mercury lamps. As it is a solid-state technology, it has a customizable emission wavelength, long life time, low voltage operation, instant on/off, it is shock resistant, easy to integrate (design flexibility, simple driving circuits), and ecology friendly (no hazardous substance such as mercury, no ozone production). In addition, in recent years performance of Deep UV LED has increased markedly. At NIKKISO, we have overcome many issues plaguing the industry, like output power and lifetime for UVB (280-320 nm) and UVC (<280 nm) LEDs. In this presentation we will review the technical progress that has been made recently, we will present the DUV lighting solutions of Nikkiso (SMD package, Modules), and we will discuss the potential markets penetration including spectroscopy applications in analytical and life sciences instrumentation
Yassine H. Benchekroun
Yassine H. Benchekroun Sidi Mohamed Ben Abdallah University, Morocco
Title: Uncertainty profile; a global strategy for validation and estimation of uncertainty: Application to a high-performance thin-layer chromatographic method
Biography:
Yassine Hameda Benchekroun is a PhD candidate in Chemometrics; His research has focused on several lines: development of methodologies for pharmaceutical and biomedical analysis, and quality assurance for drug assays; chemometric pattern recognition covering methodological developments; experimental design and optimization of the analytical process; validation of analytical methods and estimation of the measurement uncertainty.
Abstract:
The aim of this work is to apply a new technique for the validation of quantitative analytical procedures based on β-content tolerance interval and uncertainty profile. Also, an original strategy for estimating measurement uncertainty by the same approach has been developed. The performance of our proposal was confirmed by application to a High Performance thin-layer chromatography for formulation drugs. Compared to the classical strategy, the new approach has a more holistic character. It means that it is no longer necessary to know the various individual steps into which the analytical method can be broken down since this latter is taken as a whole.
Natalia Szczepańska
Gdańsk University of Technology, Poland
Title: Assessment of toxic effect and endocrine potential of food packages extracts
Biography:
Natalia Szczepańska has completed her MSc studies in 2014 from Chemical Faculty Gdansk University of Technology. She started PhD studies on Department of Analytical Chemistry in Gdansk University of Technology in 2014. She has published 3 papers in reputed journals and 1 monograph.
Abstract:
In the scientific literature related to the widely understood issue of packaging materials designed to have contact with food there is much information on raw materials used for their production, their physiochemical properties, types and parameters. There are also a great number of publications on the management and disposal of used packaging. Unfortunately, not much attention is given to the matters concerning migration of toxic substance from packaging and its actual influence on the body of the final consumer of the food packed, even though health protection and food safety are the priority tasks. The main criterion for approving packaging materials for contact with food are the toxic properties of the substances used to produce a given material and the degree of global migration and specific migration. Unfortunately, however, the standard guidelines for assessing the impact of packaging on food quality only take into account the numerical values of these parameters fixed for a small group of compounds. Such approach raises many objections, given that the results of a recent study showed that apart from the monitored compounds leaching from the surface of the package, there are also other contaminants and their derivatives formed by interaction with food components and as a result of technological procedures applied. The goal of tests was to estimate the impact of foodstuff packaging type, production and storage conditions on the degree of leaching of potentially toxic and endocrine active compounds to foodstuffs with the use of the acute toxicity test Microtox® and endocrine potential YES/YAS.