Bonnie Tay-Jones Yen Ping
Malaysian Palm Oil Board, Malaysia
Title: Gas chromatography with flame ionization detection of 1,4-dioxane in palm-based fatty alcohol ethoxylates
Biography
Biography: Bonnie Tay-Jones Yen Ping
Abstract
1,4-Dioxane, a toxic by-product may be formed during the ethoxylation of fatty alcohol. A simple and rapid method using gas chromatography with flame ionization detector (GC-FID) was developed to detect-1,4-dioxane in commercial palm-based fatty alcohol ethoxylate (FAEO). This method involved spiking of 1,4-dioxane into FAEO samples, and directly injecting the spiked samples into GC-FID. The method was validated according to the ICH harmonized tripartite guideline. The calibration curves for 1,4-dioxane showed good linearity with correlation coefficient of 0.9999. The accuracy of the method was indicated by recovery obtained for spiked 1,4-dioxane samples at 5 levels of spiking, i.e. at 30, 60, 100, 200 and 500 μg/g, where recoveries were within 99 – 105% with relative standard deviation (RSD) of less than 4.0%. The RSD values of the intra-day and inter-day precision were less than 1.0%. The limit of detection and quantification was 10 μg/g and 30 ug/g, respectively. The identity of 1,4-dioxane recovered from the palm-based fatty alcohol ethoxylate was confirmed by a GC-mass spectrometer detector.