Hamid Hashemi-Moghaddam
Islamic Azad University, Iran
Title: Molecularly imprinted layer-coated silica nanoparticles toward highly selective separation of active glycine from urine sample
Biography
Biography: Hamid Hashemi-Moghaddam
Abstract
This work reports the preparation of glycine imprinted polymer layer-coated silica nanoparticles toward analysis of trace glycine in complicated matrices. To induce the selective occurrence of surface polymerization, the polymerizable double bonds were first grafted at the surface of silica nanoparticles by the silylation. Afterwards, the glycine templates were imprinted into the polymer-coating layer through the interaction with functional monomers. The programmed heating led to the formation of uniform glycine-imprinted polymer layer with controllable thickness, and further improved the reproducibility of rebinding capacity. After removal of templates, recognition sites of glycine were exposed in the polymer layers. As a result, the maximum rebinding capacity was achieved with the use of optimal grafting ratio. There was also evidence indicating that the glycine-imprinted polymer nanoparticles compared with non-imprinted polymer nanoparticles had a higher selectivity and affinity to glycine. Moreover, using the imprinted particles as dispersive solid phase extraction (DSPE) materials, the recoveries of glycine determined by UV-Vis spectrophotometry were 84.6% in the spiked urine sample. These results show the possibility that the highly selective separation and enrichment of trace glycine from urine sample can be achieved by the molecular imprinting modification at the surface of silica nanoparticles.