Mohammed Farrag El-Behairy
National Research Centre, Egypt
Title: Enantiomeric separation of biologically active 2-Pyrazolines on Lux Cellulose-2 and Lux Amylose-2: A comparative study
Biography
Biography: Mohammed Farrag El-Behairy
Abstract
The enantioselective resolution of a set of biologically active 3-(benzo[d][1,3]dioxol-5-yl)-5-tert-butyl-4,5-dihydropyrazole derivatives (1-21) is demonstrated. Two chiral stationary phases based on the chloromethylphenylcarbamate derivatives of amylose or cellulose: namely Lux Cellulose-2 and Lux Amylose-2, were evaluated for the chiral separation of theses 2-pyrazolines using enantioselective high performance liquid chromatography (HPLC). Different mobile phases and separation modes have been investigated supported by the versatility of Lux columns. Baseline separations of all investigated compounds have been achieved using different mobile phases such as (pure Methanol, Acetonitrile, or Ethanol, or Mixtures of Methanol/Acetonitrile, Acetonitrile/Ethanol, Hexane/Ethanol). In general, Cellulose-2 column showed better enantio-separation for most of the compounds represented by high resolution in shorter time. For example compound 4 (R = H) showed Rs = 20 and run time 36 min when using Acetonitrile 100% on lux cellulose-2 while using Methanol/Acetonitrile 1/1 v/v showed Rs = 8 and run time up to 6.0 min (Rs = 1.40 for amylose-2).