Sigrid Mennickent
University of Concepción, Chile
Title: Quantitative determination of Topiramate in human serum and umbilical cord blood
Biography
Biography: Sigrid Mennickent
Abstract
A simple, rapid, specific, precise and accurate LC/DAD method for quantification of topiramate in human serum and cordon umbilical cord blood was developed and validated. The method includes a liquid-liquid extraction of the analyte from the both matrix using a dichloromethane as extraction solvent. Dabsil was used as rderivatization compound. In the developed method, chromatography was performed using acetonitrile: acetate buffer (60:40, v/v) as mobile phase, with a flow of 1 mL/min, and Purosphere ® C10 column (5 uµ). Detection was done at 254 nm. The regression data for the calibration plots showed good linear relationship (r=0.999) for both matrix, in the range of 2.0 and 40.0 ug/mL, in human serum (therapeutic range concentration: 5.0-20.0 ug/mL), and between 1.6 and 50.0 ug/mL for umbilical cord blood. The % RSD of intra-assay and inter-assay precision, were in the range of 0.98% to 2.97% (n=3) and 1.06% to 3.54% (n=9), respectively, for human serum, and 1.21% to 3.58% (n=3) and 2.10% and 3.68% (n=9), for umbilical cord blood. Recovery values were between 94.72% and 98.96% (RSD ≤4.25), for both matrix, respectively. Patient serum samples and umbilical cord blood samples were analyzed by this method successfully. Therefore, this LC/DAD method is suitable for quantitative determination of topiramate in these biological fluids.