Miranda Younes
University in Alexandria, Egypt.
Title: Comparative study of two Validated Stability-indicating chromatographic methods applied for simultaneous determination of Coenzyme Q10 and Vitamin E in combined tablet dosage form
Biography
Biography: Miranda Younes
Abstract
Two simple, precise and stability-indicating High-Performance Liquid Chromatography (HPLC) and High-Performance Thin-Layer Chromatographic (HPTLC) methods were introduced and compared for the simultaneous determination of Coenzyme Q10 (Q) and Vitamin E (E) ; alpha-tocopherol acetate, in tablet formulation. The HPLC separation was achieved on a C18 column using Acetonitrile: Tetrahydrofuran: Water (55:40:5, v/v/v) as mobile phase at a flow rate of 0.7 mL/min at ambient temperature. The quantitation was achieved UV detection at 280 nm over the concentration range 5 – 60 and 50 - 200 μg/mL for Q and E, respectively. For HPTLC method, Q and E were chromatographed on silica Gel 60 F254 TLC plate using Benzene: Chloroform (7: 3, % v/v) as mobile phase and then scanned at 280 nm. Linearity is in the range of 0.3 - 2 and 0.5 – 5 μg/band for Q and E, respectively. The two methods were validated according to ICH guidelines and different chromatographic parameters were optimized for adequate determination of Q and E in the tablet. Furthermore, a forced degradation study of Q and E was done under various conditions including; hydrolysis (acid, alkaline and neutral), oxidation, thermal and photo-decomposition. Statistical analysis proved that the two methods were precise, accurate, selective and economical with no significant difference between them. They may be used for routine simultaneous estimation of Q and E in tablets.