Kamlesh Shirvas
Guru Ghasidas University, India
Title: MALDI-MS imaging of low molecular weight biomolecules in mouse brain using TiO2 nanoparticles
Biography
Biography: Kamlesh Shirvas
Abstract
The presence of low molecular weight biomolecules in brain has important role in biosynthesis, product degradation, energy production, signaling and defense. Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) has proven to be one of the most powerful tools available to researchers in biochemistry and molecular biology for the identification of biomolecules in various types of biological samples. We report the detection of a group of endogenous low molecular weight metabolites in mouse brain (80-500 Da) using TiO2 nanoparticles (NPs) without any washing and separation step prior to MS analysis. This approach is a simple, inexpensive, washing and separation free for imaging and identification of low molecular weight biomolecules in mouse brain. The white and gray matter of the mouse brain is differentiated by the ion distribution pattern of biomolecules found in the tissue section. We believe that the biochemical information from distinct regions of the brain using a MALDI-MS imaging will be helpful in elucidating the imbalances linked with diseases in biomedical samples.