Lihan Tan
Bioprocessing Technology Institute, Singapore
Title: Measurement of histones in mammalian cell culture harvest
Biography
Biography: Lihan Tan
Abstract
Histones, in the form of chromatin heteroaggregates in cell culture harvests, are becoming recognized as a major challenge in antibody purification. However, recent publications have shown that immunoassays for host cell proteins do not detect histones. In addition, histone standards provided in commercially available histone ELISA kits are not based on authentic CHO histones. This presentation will show how to prepare authentic host cell histone standards, and how they are necessary for accurate quantitation of histone contamination in cell culture harvests and during purification procedures.