Ashraf Ali
Department of Chemistry, Inha University, South Korea
Title: Sub-2μm C18 Bound Silica Monolith Particles as HPLC Stationary Phase of High Separation Efficiency
Biography
Biography: Ashraf Ali
Abstract
Sub-2μm porous silica monolith particles have been prepared successfully prepared by sol-gelprocess followed by grinding and calcinations at 550ËšC. The particles were derivatized with a C18 ligand followed by end-capping with a mixture of hexamethyldisilazane (HMDS), andtrimethylchlorosilane (TMCS). The resultant phase was packed in glass lined stainless steel microcolumn is much better than that of commercial C-18 phase. This phase has shown some encouraging possibility for fast analysis when packed in a short column. This study offers a promising vision towards commercialization of chromatographic phases based on silica monolith particles.
Graphical abstract
Fig.1. Microscopic view of silica monolith particles (a) and SEM images of C18-bound silicamonolith particles with different magnification (b, c, d). The scale bars are 20, 10, and 1 μm forc, and d, respectively. 0 5 10 15 20 25 30 min
Fig.2. Chromatogram C18-bound sub-2μm silica monolith particles of current study. The mobilephase and column dimension is 60/40 acetonitrile/water, 0.1% TFA, 214nm and (1mm ID x 300m m length). The analytes elusion order is: Phenol, Acetophenone, 4-Methyl-2-nitroanailine, Benze ne and Toluene