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S. KUMAR

S. KUMAR

Uppsala University, Sweden

Title: Infrared spectroscopy combined with imaging modalities is a new technique to understand the disease pathology

Biography

Biography: S. KUMAR

Abstract

Methodology: Development of modern infrared spectroscopy has a wide range of biological applications. Initially, it was extensively used for protein secondary structure analysis as well as nucleotides, lipids and carbohydrates. Now with time it extended to biodiagnositic tools such as cells, tissues and bio-fluids. Infrared imaging can use to discriminate between healthy and diseased one. IR microscope equipped with FPA (focal plane array) detector able to scan the larger area with quick time and that helps to measures the cells as well as tissue (histopathology). An IR synchrotron light source connected with IR microscope further enhances the spatial resolution at diffraction limit. I will present briefly the use of this method of infrared spectroscopy in disease pathology with two examples (breast cancer and multiple sclerosis).

Experimental setup: The spectroscopic imaging data on breast cancer and multiple sclerosis samples were acquired in transmission on deparaffined 3-5 μm thick tissue slices deposited on 40x26 mm2 BaF2 slides. For cells, the fibroblasts were grown on CaF2 window and directly used for FTIR measurements. We used a Hyperion imaging system (Bruker) equipped with a 64*64 MCT (Mercury-Cadmium-Telluride) FPA (Focal Plane Array) detector.

Results: Using FTIR imaging technique to discriminate healthy and diseased samples on the basis of chemical changes due to its potential to probe tissues and cells at the molecular level. Now with the application of advanced focal plane array detector able to scan large area of samples in a short time, helps to investigate the specific changes that could be correlated with the pathology and different environmental stresses.

Acknowledgement: We are grateful to CIHR-THRUST (Canada) postdoctoral fellowship, Brain Back to Brussels (Belgium) and VINNOVA-VINNMER mobility for growth grant to S Kumar.